基于转录组的水稻‘甬籼15’早熟机制解析  

Transcriptome analysis of the early maturity mechanism of the rice cultivar‘Yongxian 15’

作　　者：张雨洁 杨繁婧 应泉盛 姜洁锋 叶楚玉 施贤波 ZHANG Yujie;YANG Fanjing;YING Quansheng;JIANG Jiefeng;YE Chuyu;SHI Xianbo(College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,Zhejiang,China;Institute of Crop Research,Ningbo Academy of Agricultural Sciences,Ningbo 315040,Zhejiang,China)

机构地区：[1]浙江大学农业与生物技术学院,浙江杭州310058 [2]宁波市农业科学研究院作物研究所,浙江宁波315040

出　　处：《浙江大学学报(农业与生命科学版)》2025年第2期228-239,共12页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：浙江省水稻新品种选育重大科技专项(2021C02063-4)。

摘　　要：‘甬籼15’是浙江省主推的早熟早籼稻品种,解析其早熟分子机制对早熟水稻育种具有重要意义。本研究对‘甬籼15’(早熟)及‘中早39’(中熟)在幼穗分化关键时期的转录组数据进行了系列分析。通过计算不同日期转录水平的皮尔逊相关系数,发现在水稻幼穗分化前后大量基因发生了差异表达。筛选阶段性差异表达的振荡基因发现,与茉莉酸(jasmonic acid, JA)合成及细胞壁合成有关的基因得到富集。时序聚类分析表明,JA合成及信号通路相关基因被特异性富集到了在幼穗分化开始后表达上调并在幼穗分化Ⅱ期后表达下降的基因簇中。通过比较振荡基因的表达模式,推测JA信号通路参与调控‘甬籼15’幼穗分化,并发现OsGI、OsFD1、RFL、OsMADS15、OsHESO1等基因可能通过影响‘甬籼15’的光周期通路促进其幼穗分化。研究结果为早熟水稻分子设计育种提供了新靶点。‘Yongxian 15’is a leading early-maturing early indica rice cultivar in Zhejiang Province,and elucidating its early maturity molecular mechanisms is of great significance for the breeding of early-maturing rice.In this study,we conducted a series of transcriptome analyses on‘Yongxian 15’(early-maturing)and‘Zhongzao 39’(medium-maturing)during critical stages of spikelet differentiation.By calculating the Pearson correlation coefficient between the transcriptomes at different dates,a large number of genes were found to be differential expressions before and after spikelet differentiation.Oscillatory genes that were stage-differentially expressed were filtered out,and the genes related to jasmonic acid(JA)biosynthesis and cell wall synthesis were enriched.Temporal clustering analysis demonstrated that JA biosynthesis-and signaling pathway-related genes were specifically enriched in a gene cluster exhibiting upregulated expressions after the initiation of spikelet differentiation and downregulated expressions following stageⅡof spikelet differentiation.Comparing the expression patterns of specific oscillatory genes,it was assumed that JA signaling pathway was involved in regulating spikelet differentiation in‘Yongxian 15’.A series of genes,including OsGI,OsFD1,RFL,OsMADS15,and OsHESO1,were identified as potential regulators influencing the photoperiod pathway to promote spikelet differentiation in‘Yongxian 15’.These findings provide novel targets for the molecular breeding of early-maturing rice cultivars.

关 键 词：水稻 ‘甬籼15’ 早熟 幼穗分化 转录组 

分 类 号：S511.31[农业科学—作物学]