基于TLR4、NF-κB探讨温针灸对兔膝骨关节炎软骨的影响  

Intervention Mechanism of Warm Acupuncture on TLR4 and NF-κB Signaling Pathway in Rabbit Model of Knee Osteoarthritis

作　　者：张茂[1] 金学连 张文欣 赵婷 ZHANG Mao;JIN Xuelian;ZHANG Wenxin;ZHAO Ting(Department of Traditional Chinese Medicine,The First People's Hospital of Yinchuan,the Second Clinical Medical College of Ningxia Medical University,Yinchuan 750001,China;Yinchuan Hospital of Traditional Chinese Medicine,Yinchuan 750001,China;School of Traditional Chinese Medicine,Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]银川市第一人民医院中医科,宁夏医科大学第二临床医学院,银川750001 [2]银川市中医医院,银川750001 [3]宁夏医科大学中医学院,银川750004

出　　处：《宁夏医科大学学报》2025年第3期249-253,共5页Journal of Ningxia Medical University

基　　金：宁夏自然科学基金项目(2022AAC03739)。

摘　　要：目的观察温针灸对兔膝骨关节炎模型软骨组织的Toll样受体4(TLR4)、核因子κB(NF-κB)表达及软骨细胞凋亡的影响。方法将40只新西兰大白兔随机分为空白组、模型组、双氯芬酸钠组和温针灸组,每组10只。采用关节制动法造模。空白组和模型组不予任何治疗,温针灸组每天于患侧鹤顶、后三里、内外膝眼、阳陵泉穴位处予以温针灸治疗1次,双氯芬酸钠组给予15 mg·kg^(-1)双氯芬酸钠灌胃。采用HE染色观察病理变化,Western blot检测软骨组织中TLR4、NF-κB蛋白表达水平,TUNEL染色检测软骨细胞凋亡情况。结果与模型组对比,双氯芬酸钠组和温针灸组的TLR4、NF-κB的蛋白表达水平均降低(P均<0.05),软骨细胞凋亡率差异无统计学意义(P>0.05);与双氯芬酸钠组比较,温针灸组软骨细胞凋亡率降低(P<0.05),而TLR4、NF-κB的表达水平差异均无统计学意义(P均>0.05)。结论温针灸可抑制兔膝骨关节炎的TLR4、NF-κB的表达,抑制软骨细胞的凋亡,减轻膝骨关节炎软骨的炎症反应。Objective To observe changes in Toll-like receptor 4(TLR4),NF-κB and chondrocyte apoptosis in a rabbit model of knee osteoarthritis after warm acupuncture.Methods A total of 40 New Zealand white rabbits were randomly divided into four groups,blank group,model group,diclofenac sodium group and warm acupuncture group,with 10 rabbits in each group.Joint Braking Method was used to make models.The blank group and the model group did not receive any treatment.The warm acupuncture and moxibustion group was treated with warm acupuncture and moxibustion once a day at Heding,Housanli,inner and outer knee eyes and Yanglingquan of the affected side,while the diclofenac sodium group was given 15 mg·kg^(-1) diclofenac sodium by gavage.HE staining was used to observe pathological changes,Western blot was used to detect the expression of TLR4 and NF-κB protein levels in cartilage tissue,and TUNEL staining was used to detect chondrocyte apoptosis.Results Compared with the model group,the expression levels of TLR4 and NF-κB in diclofenac sodium group and warm acupuncture and moxibustion group decreased(P all<0.05),and there was no significant difference in the apoptosis rate of chondrocytes(P>0.05).Compared with diclofenac sodium group,the apoptosis rate of chondrocytes in warm acupuncture group decreased(P<0.05),while the expression levels of TLR4 and NF-κB were no significantly different(P all>0.05).Conclusion Warm acupuncture and moxibustion can inhibit the expression of TLR4,NF-κB,and the apoptosis of chondrocytes,and reduce the inflammatory reaction of knee osteoarthritis.

关 键 词：膝骨关节炎 温针灸 TLR4蛋白 NF-ΚB蛋白 

分 类 号：R392.9[医药卫生—免疫学]