分光光度法测定甲磺酸酚妥拉明注射液中焦亚硫酸钠的含量  

Determination of content of sodium pyrosulfite in phentolamine mesylate injection by UV-VIS Method

作　　者：王皖 李钰鑫 陈鸿玉 王晓菲 Wang Wan;Li Yuxin;Chen Hongyu;Wang Xiaofei(Hunan Institute for Drug Control,Changsha 410001,China)

机构地区：[1]湖南省药品检验检测研究院,湖南长沙410001

出　　处：《煤炭与化工》2025年第4期148-151,160,共5页Coal and Chemical Industry

摘　　要：为了建立分光光度法测定甲磺酸酚妥拉明注射液中焦亚硫酸钠的含量,采用分光光度法,以0.01%EDTA溶液为溶剂,加入0.05%碱性品红溶液2 mL,0.3%甲醛溶液3 mL,摇匀,显色反应20 min,556 nm测定吸光度值,外标法计算含量。结果表明,焦亚硫酸钠在0.12696~1.9044μg/mL线性关系良好(r=0.9999),平均回收率101.01%(n=9,RSD=0.8%);显色溶液在1 h内完成测定;重复性结果良好。建立的方法简便、准确、专属性良好,可用于甲磺酸酚妥拉明注射液中焦亚硫酸钠的测定。The absorbance value was measured at 556 nm,and the content was calculated by external standard,.in order to establish UV-VIS method for the determination of content of Na2S2O5 in phentolamine mesylate injection by using UV-VIS method with 0.01%EDTA solution as solvent,adding 2 mL of 0.05%basic fuchsin and 3 mL of 0.3%formaldehyde solution,shaking well,color reaction for 20 min.Results showed that Na2S2O5 had good linear relationship within the range of 0.12696~1.9044μg·mL^(-1)(r=0.9999),its average recovery rate was 101.01%,with an RSD of 0.8%(n=9).The solution was determined within 1 h.Repeatability with good results.The established method is simple,accurate and good exclusivity and suitable for the determination of Na2S2O5 content in phentolamine methylate injection.

关 键 词：分光光度法 甲磺酸酚妥拉明注射液 焦亚硫酸钠 含量测定 

分 类 号：O657.32[理学—分析化学]