全真一气汤调控PIK3R3、PLA2G10、TGFBR1、BIRC5的表达水平抑制COPD向肺癌发展的机制研究  

Exploring the mechanism of Quanzhen Yiqi Decoction(全真一气汤)in inhibiting COPD progression to lung cancer by regulating the expression levels of PIK3R3,PLA2G10,TGFBR1 and BIRC5

作　　者：赵佳佳 陈可强 蔡颖利 吴伟斌 王春娥[2] ZHAO Jia-jia;CHEN Ke-qiang;CAI Ying-li;WU Wei-bin;WANG Chun-e(Fujian University of Traditional Chinese Medicine,Fuzhou 350003,China;Second Affiliated Hospital of Fujian University of Chinese Medicine,Fuzhou 350003,China)

机构地区：[1]福建中医药大学,福建福州350003 [2]福建中医药大学附属第二人民医院,福建福州350003

出　　处：《时珍国医国药》2025年第6期1023-1028,共6页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金面上项目(81774097);福建省自然科学基金(2020J01251)。

摘　　要：目的观察全真一气汤对慢性阻塞性肺疾病大鼠肺组织PIK3R3、PLA2G10、TGFBR1和BIRC5表达水平,探究对其肺癌发生率的影响。方法将18只SD大鼠随机分为中药组和模型组,每组各9只。利用烟熏联合内毒素脂多糖(LPS)构建COPD大鼠模型。模型组每日予0.9%生理盐水灌胃;中药组每日予40%全真一气汤浓缩汤剂灌胃。利用PCR技术检测大鼠肺组织PIK3R3、PLA2G10、TGFBR1、BIRC5 mRNA表达水平,Western blot检测大鼠肺组织中PIK3R3、PLA2G10、TGFBR1、BIRC5蛋白水平。结果中药组PIK3R3、PLA2G10、BIRC5 mRNA表达水平较模型组低,其表达丰度分别为模型组的0.159、0.138、0.647倍;中药组TGFBR1 mRNA表达水平较模型组高,其表达丰度为模型组的1.867倍。中药组PIK3R3、PLA2G10、BIRC5蛋白表达较模型组下调,中药组TGFBR1蛋白表达较模型组上调。结论全真一气汤通过调节PIK3R3、PLA2G10、BIRC5、TGFBR1的表达可能抑制COPD患者肺癌的发生。Objective To observe the effects of Quanzhen Yiqi Decoction(全真一气汤,QZYQD)on the expression levels of PIK3R3,PLA2G10,TGFBR1,and BIRC5 in the lung tissues of rats with chronic obstructive pulmonary disease(COPD)and explore its impact on the incidence of lung cancer.Methods Eighteen Sprague-Dawley(SD)rats were randomly divided into the Chinese herbal medicine(CHM)group and model group,with 9 rats in each group.A COPD rat model was established by sumdging combined with lipopolysaccharide(LPS).The model group was gavaged with daily intragastric administration of 0.9%normal saline(NS),while the CHM group was gavaged with daily intragastric administration of 40%concentrated QZYQD.PCR was used to detect mRNA expression levels of PIK3R3,PLA2G10,TGFBR1,and BIRC5,and Western blot(WB)was performed to measure protein levels in the lung tissues of rats.Results The mRNA expression levels of PIK3R3,PLA2G10,and BIRC5 in CHM group were lower than those in the model group,with expression abundances of 0.159-fold,0.138-fold,and 0.647-fold of the model group,respectively.The mRNA expression level of TGFBR1 in CHM group was higher,at 1.867-fold of the model group.Protein expressions of PIK3R3,PLA2G10 and BIRC5 were down-regulated in CHM group compared to the model group,while TGFBR1 protein expression was up-regulated.Conclusion QZYQD may inhibit the onset of lung cancer in COPD by modulating the expressions of PIK3R3,PLA2G10,BIRC5 and TGFBR1.

关 键 词：全真一气汤 慢性阻塞性肺疾病 肺癌 磷酸肌醇3激酶调控亚基3 重组人分泌磷脂酶A2-X 转化生长因子β受体Ⅰ 杆状病毒凋亡抑制蛋白5 

分 类 号：R285.5[医药卫生—中药学]