百杏润肺化浊解毒汤对哮喘模型小鼠气道炎症的影响  

Exploring the effect of Baixing Runfei Huazhuo Jiedu Decoction(百杏润肺化浊解毒汤)on airway inflammation in asthmatic model rats

作　　者：黄玲玲 王基传 赵男 王红霞 裴林 孙国强 方朝义 HUANG Ling-ling;WANG Ji-chuan;ZHAO Nan;WANG Hong-xia;PEI Lin;SUN Guo-qiang;FANG Chao-yi(Hebei University of Chinese Medicine,Shjiazhuang 050091,China;Hebei Academy of Chinese Medical Sciences,Shijiazhuang 050031,China;Hebei Provincial Key Laboratory of Turbid Toxins Syndrome,Shijiazhuang 050031,China;Hebei Key Laboratory of Integrated Chinese and Western Medicine for Lung Disease Research,Shijiazhuang 050091,China)

机构地区：[1]河北中医药大学,河北石家庄050091 [2]河北省中医药科学院,河北石家庄050031 [3]河北省浊毒证重点实验室,河北石家庄E050031 [4]河北省中西医结合肺病研究重点实验室,河北石家庄050091

出　　处：《时珍国医国药》2025年第6期1035-1039,共5页Lishizhen Medicine and Materia Medica Research

基　　金：河北省中医药管理局科研项目(2020153);河北省自然科学基金项目(H2019423092)。

摘　　要：目的探讨百杏润肺化浊解毒汤对卵清蛋白诱导的哮喘模型小鼠气道炎症及相关血清细胞因子的影响。方法60只BALB/c小鼠随机分为正常组、模型组、孟鲁司特钠组和百杏润肺化浊解毒汤低、中、高剂量组,每组10只。除正常组以0.9%氯化钠溶液代替外,其余各组小鼠均采用卵清蛋白(OVA)致敏并滴鼻激发以诱导建立哮喘模型。百杏润肺化浊解毒汤低、中、高剂量组分别予12.95,25.90,51.80 g·kg^(-1)·d^(-1)灌胃;孟鲁司特钠组予1.3 mg·kg^(-1)·d^(-1)灌胃;正常组和模型组均予0.2 mL 0.9%氯化钠溶液灌胃,各组给药均在滴鼻激发前,每日1次,连续7d。采用HE染色法观察小鼠肺组织气道炎症等病理改变;采用PAS染色法观察小鼠气道杯状细胞化生及黏液分泌情况;ELISA法检测小鼠血清白细胞介素(IL)-6、IL-13及肿瘤坏死因子(TNF)-α含量。结果与正常组比较,模型组小鼠气道及其周围有大量炎症细胞浸润,气道内可见明显杯状细胞化生和黏液分泌,血清IL-6、IL-13及TNF-α含量升高(P<0.01);与模型组比较,百杏润肺化浊解毒汤各剂量组和孟鲁司特钠组小鼠肺组织气道炎症细胞浸润、黏液分泌及杯状细胞化生程度均得到不同程度的减轻,百杏润肺化浊解毒汤高剂量组和孟鲁司特钠组小鼠血清IL-6、IL-13及TNF-α含量降低(P<0.05或P<0.01),百杏润肺化浊解毒汤低剂量组小鼠血清IL-6、IL-13含量降低(P<0.05或P<0.01),百杏润肺化浊解毒汤中剂量组小鼠血清IL-13含量降低(P<0.01),其中百杏润肺化浊解毒汤中剂量组小鼠血清IL-6含量较高于孟鲁司特钠组和百杏润肺化浊解毒汤高剂量组(P<0.05)。结论百杏润肺化浊解毒汤可能通过降低细胞因子的表达水平,进而降低由细胞因子介导的气道炎症及黏液高分泌,从而缓解哮喘症状。Objective To investigate the effect of Baixing Runfei Huazhuo Jiedu Decoction(百杏润肺化浊解毒汤,BRHJD)on the airway inflammation and related serum cytokines in ovalbumin(OVA)-induced asthmatic model mice.Methods Sixty BALB/c mice were randomly divided into six groups:the normal group,model group,montelukast sodium group,and BRHJD low/medium/high-dose groups,with 10 mice in each group.Except for the normal group replaced with 0.9%sodium chloride solution,all other groups were sensitized with OVA and stimulated with nasal drops to induce the establishment of an asthma model.All BRHJD groups were administered via gavage at doses of 12.95,25.90,and 51.80 g·kg^(-1)·d^(-1),respectively;the montelukast sodium group received 1.3 mg·kg^(-1)·d^(-1)by gavage;while the normal and model groups were given 0.2 mL of 0.9%sodium chloride solution by gavage.All treatments were administered once daily for 7 consecutive days before stimulation by dripping nose.The pathological changes in airway inflammation in the lung tissues of mice were observed by Hematoxylin-eosin(HE)staining.The airway goblet cell hyperplasia and mucus secretion in mice were observed by periodic acid-Schiff(PAS)staining.Content of serum interleukin(IL)-6,IL-13 and tumor necrosis factor(TNF)-αwere detected by enzyme-linked immunosorbent assay(ELISA).Results Compared with the normal group,the model group showed significant airway inflammation,goblet cell metaplasia,mucus hypersecretion,and increased contents of serum IL-6,IL-13,and TNF-α(P<0.01).Compared with the model group,all BRHJD dose groups and the montelukast sodium group exhibited reduced airway inflammation,mucus secretion,and goblet cell metaplasia in varied degrees.IL-6,IL-13,and TNF-αcontents were decreased in the BRHJD high-dose and montelukast sodium groups(P<0.05 or P<0.01).The BRHJD low-dose group showed reduced contents of serum IL-6 and IL-13(P<0.05 or P<0.01),while the medium-dose group showed reduced contents of serum IL-13(P<0.01).Among them,the BRHJD medium-dose group exhib

关 键 词：百杏润肺化浊解毒汤 支气管哮喘 气道炎症 白细胞介素6 白细胞介素13 肿瘤坏死因子α 

分 类 号：R285.5[医药卫生—中药学]