通络三生饮酊剂缓解癌性疼痛的作用及对IL-1β、TNF-α和PGE2的影响  

On the effect of Tongluo Sanshengyin Tincture(通络三生饮酊剂)on alleviating cancer pain and its impact on IL-1β,TNF-αand PGE2

作　　者：孙民朋 施艾每 徐方园 贾金虎 郭伟 孙宏新[3] SUN Min-peng;SHI Ai-mei;XU Fang-yuan;JIA Jin-hu;GUO Wei;SUN Hong-xin(School of Traditional Chinese Medicine of Hong Kong Baptist University,Hong Kong 999077,China;School of the First Clinical Medicine of Henan University of Chinese Medicine,Zhengzhou 450046,China;First Affiliated Hospital of Henan University of Chinese Medicine,Zhengzhou 450000,China)

机构地区：[1]香港浸会大学中医药学院,中国香港999077 [2]河南中医药大学第一临床医学院,河南郑州450046 [3]河南中医药大学第一附属医院,河南郑州450000

出　　处：《时珍国医国药》2025年第6期1045-1049,共5页Lishizhen Medicine and Materia Medica Research

基　　金：河南省中医药重点学科建设项目(豫卫中医药科教[2014]1号);张仲景传承与创新专项(GZY-KJS-20220382);河南省中西医学免疫工程研究中心(豫发改高技【2020】701号)。

摘　　要：目的通过构建小鼠癌痛模型,探究通络三生饮酊剂外治癌性疼痛的作用机制。方法于KM小鼠右腿坐骨神经周围肌肉处接种S180细胞,通过瘤体压迫坐骨神经制备癌痛模型,并随机分为模型组、通络三生饮高、低剂量组和对照组,每组10只,另设10只健康KM小鼠作为空白组,每组小鼠雌雄各半。造模成功后开始给药,连续给药12d。治疗前后利用热板仪和Von Frey纤维丝分别检测小鼠热痛阈值及机械性痛阈值,Elisa法检测血清及瘤体中白介素-1β(IL-1β)、肿瘤坏死因子α(TNF-α)和前列腺素2(PGE2)含量。结果与空白组相比,模型组、通络三生饮高、低剂量组和对照组小鼠热痛阈值和机械性痛阈值均于造模后第8天降低(P<0.01),且无组间差异(P>0.05),治疗结束后,与模型组相比,通络三生饮高、低剂量组小鼠热痛阈值和机械性痛阈值升高(P<0.01,P<0.05),血清中IL-1β、TNF-α及PGE2含量降低(P<0.01,P<0.05)。结论通络三生饮酊剂能够缓解小鼠癌痛,其机制可能通过抑制外周炎症因子水平发挥作用。Objective To investigate the mechanism of Tongluo Sanshengyin Tincture(通络三生饮酊剂,TST)in treating the cancer pain by establishing a mouse model of cancer pain.Methods S180 cells were inoculated into the muscle around the right sciatic nerve of KM mice to establish a cancer pain model via tumor compression of the sciatic nerves.The mice were randomly divided into the model group,high-dose TST group,low-dose TST group,and control group(10 mice per group),with an additional 10 healthy KM mice serving as the blank group.All groups contained half male and half female mice.After successful modeling,the drug was administered for 12 consecutive days.Before and after treatment,the thermal pain threshold and mechanical pain threshold of mice were detected by hot plate instrument and Von Frey filaments,and the contents of IL-1β,TNF-αand PGE2 in serum and tumor were detected by ELISA.Results Compared with the blank group,the thermal pain threshold and mechanical pain threshold of mice in the model group,high-dose TST group,low-dose TST group,and control group were all decreased on the 8th day after modeling(P<0.01),without differences between groups(P>0.05).After treatment,both high-dose and low-dose TST groups exhibited increased thresholds of thermal pain and mechanical pain(P<0.01,P<0.05)and reduced contents of IL-1β,TNF-α,and PGE2 in serum(P<0.01,P<0.05),compared with the model group.Conclusion TST can alleviate cancer pain in mice,and its mechanism may be related to inhibiting peripheral inflammatory factor levels.

关 键 词：通络三生饮酊剂 癌性疼痛 炎症因子 白介素-1Β 肿瘤坏死因子-α 前列腺素2 

分 类 号：R285.5[医药卫生—中药学]