HPLC指纹图谱和多成分定量结合化学模式识别法对地骨皮饮片的质量评价  

Quality evaluation of Lycii Cortex slices(地骨皮饮片)by HPLC fingerprint and multi-component quantification combined with chemical pattern identification

作　　者：刘宇婷 赵梦薇 王玉霞 王杰敏 裴林 宋永兴 马东来 LIU Yu-ting;ZHAO Meng-wei;WANG Yu-xia;WANG Jie-min;PEI Lin;SONG Yong-xinngg;MA Dong-lai(School of Pharmacy,Hebei University of Chinese Medicine,Shijiazhuang 050200,China;Hebei Academy of Chinese Medical Sciences,Shijiazhuang 050031;Hebei Provincial Innovation Center for Processing Technology of Traditional Chinese Medicine,Shijiazhuang 050091;Hebei Provincial International Joint Research Center for Utilization and Quality Evaluation of Traditional Chinese Medicine Resources,Shijiazhuang 050091,China)

机构地区：[1]河北中医药大学药学院,河北石家庄050200 [2]河北省中医药科学院,河北石家庄050031 [3]河北省中药炮制技术创新中心,河北石家庄050091 [4]河北省中药资源利用与质量评价国际联合研究中心,河北石家庄050091

出　　处：《时珍国医国药》2025年第6期1070-1075,共6页Lishizhen Medicine and Materia Medica Research

基　　金：道地药材可持续利用(202400262157-3);河北省中医药管理局科研计划项目(2024094);河北省重点研发计划项目(23372504D);河北省三期现代农业产业技术体系创新团队项目(HBCT2024110201)。

摘　　要：目的HPLC测定9种化学成分含量并建立指纹图谱,结合化学模式识别法对26批地骨皮饮片质量进行评价。方法色谱柱Zorbax C_(18)SB-AQ(250 mm×4.6 mm,5μm),流动相乙腈-0.1%三氟乙酸水溶液,进样体积10μL,柱温40℃,梯度洗脱,流速1.0 mL/min,检测波长280 nm。对地骨皮进行定量,采用聚类分析、主成分分析并计算综合得分进行排名,评价26批地骨皮饮片质量。结果建立了地骨皮饮片HPLC指纹图谱,相似度为0.958~0.998;聚类分析将饮片聚成四大类;主成分分析结果表明样品S21得分最高。结论研究建立了多指标含量测定及指纹图谱结合化学模式识别的方法,为临床中地骨皮饮片的质量评价和合理应用提供了参考。Objective To determine the contents of nine chemical components in Cortex Lycii(地骨皮)using HPLC,establish fingerprint profiles,and evaluate the quality of 26 batches of Lycii Cortex slices(地骨皮饮片)by combining chemical pattern identification.Methods The chromatography was performed on a Zorbax C_(18)SB-AQ column(250 mm×4.6 mm,5μm)with a mobile phase of acetonitrile-0.1%trifluoroacetic acid aqueous solution under gradient elution.The flow rate was 1.0 mL/min,column temperature was 40℃,injection volume was 10μL,and detection wavelength was set at 280 nm.Quantitative analysis of Cortex Lycii components was conducted.Cluster analysis(CA)and principal component analysis(PCA)were applied to calculate comprehensive scores for ranking,thereby evaluating the quality of the 26 batches.Results HPLC fingerprint profiles of Lycii Cortex slices were established with similarity values ranging from 0.958 to 0.998.CA classified the slices into four major groups.PCA results indicated that sample S21 achieved the highest comprehensive scoreConclusion A method combined with multi-component quantitative analysis and fingerprint profiling with chemical pattern identification was successfully established.This approach provides a reference for quality evaluation and rational application of Lycii Cortex slices in clinical practice.

关 键 词：地骨皮 高效液相色谱法 指纹图谱 化学模式识别 聚类分析 主成分分析 

分 类 号：R282.71[医药卫生—中药学] R284.1[医药卫生—中医学]