加味沙参麦冬汤配方颗粒与汤剂物质成分对比研究  

A comparative study on the chemical constituents of Jiawei Shashen Maidong Decoction(加味沙参麦冬汤)in form of granule and decoction

作　　者：成焕波 张营 许梦玲 石瑞雪 陈美玲 雷茜贻 殷涛 杨强 张帆 巴元明[1,2,3] CHENG Huan-bo;ZHANG Ying;XU Meng-ling;SHI Rui-xue;CHEN Mei-ing;LE Qian-yi;YIN Tao;YANG Qiang;ZHANG Fan;BA Yuan-ming(Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan 430061,China;Hubei Shizhen Laboratory,Wuhan 430065,China;Afiliated Hospital of Hubei University of Chinese Medicine,Wuhan 430061,China;Jing Brand Chizhengtang Pharmaceutical Co.,Ltd.,Hubei Provincial Engineering Technology Research Center for Traditional Chinese Medicine Formula Granules,Hubei Provincial Key Laboratory of Quality and Safety for Traditional Chinese Medicine Health Food,Huangshi 435100,China)

机构地区：[1]湖北省中医院,湖北时珍实验室,湖北武汉430061 [2]湖北时珍实验室,湖北武汉430065 [3]湖北中医药大学附属医院,湖北武汉430061 [4]劲牌持正堂药业有限公司,湖北省中药配方颗粒工程技术研究中心,中药保健食品质量与安全湖北省重点实验室,湖北黄石435100

出　　处：《时珍国医国药》2025年第6期1076-1085,共10页Lishizhen Medicine and Materia Medica Research

基　　金：湖北省重点研发计划项目(2022ACA003);黄石市揭榜制科技项目(2023A005)。

摘　　要：目的比较加味沙参麦冬汤配方颗粒(JWSSMD-k)与加味沙参麦冬汤汤剂(JWSSMD-t)物质成分的异同性。方法建立加味沙参麦冬汤UPLC-ESI-QTOF-MS/MS分析方法,结合质谱碎片、对照品、文献进行化学成分鉴定;采用PCA、OPLS-DA等多元统计分析技术对比JWSSMD-k与JWSSMD-k的异同性,筛选出影响较大指标建立含量测定方法,并比较含量的差异。结果共鉴定了36个共有峰,JWSSMD-k与JWSSMD-t正、负离子模式下总离子流图谱一致性高,化学成分差异较小;PCA不能明显区分二者差异,进一步结合OPLS-DA和t统计分析,29个成分无统计学差异,筛选出了7个差异性成分,建立了差异性成分含量测定方法,该方法稳定性良好;JWSSMD-k组中柯里拉京、没食子酸甲酯、诃子次酸、triglloylglucose、矮地茶苷J等5个成分明显高于JWSSMD-t组,而岩白菜素、苦杏仁苷则低于JWSSMD-t组。结论JWSSMD-k与JWSSMD-t物质成分整体差异性较小,二者具有整体质量一致性与特定成分差异性的特征,该研究为加味沙参麦冬汤配方颗粒临床应用提供依据。Objective To compare the similarities and differences in the material components between Jiawei Shashen Maidong Decoction(加味沙参麦冬汤,JWSSMD)in form of granule(JWSSMD-k)and its decoction(JWSSMD-t).Methods UPLC-ESI-QTOF-MS/MS analytical method was established for JWSSMD.Chemical composition was identified by combining mass spectral fragmentation,reference substances and literature.Multivariate statistical analysis techniques,including PCA and OPLS-DA,were employed to compare the similarities and differences between JWSSMD-k and JWSSMD-t.High-impact influential components were screened to establish a quantitative determination method,and content differences were compared.Results A total of 36 common peaks were identified.The total ion chromatograms of JWSSMD-k and JWSSMD-t under positive and negative ion modes showed high consistency,with minor differences in chemical components.PCA could not distinctly differentiate between the two,but further OPLS-DA and t-statistical analyses revealed no statistically differences in 29 components.Seven differential components were identified,and a stable quantitative method of differential ingredient content was established.In the JWSSMD-k group,five components(corilagin,methyl gallate,chebulic acid,triglloylglucose,and ardisiacrispin J)were significantly higher than those in JWSSMD-t groups,while bergenin and amygdalin was lower.Conclusion The overall differences in material components between JWSSMD-k and JWSSMD-t are insignificant,demonstrating both overall quality consistency and specific component variations.This study provides a basis for the clinical application of JWSSMD-k.

关 键 词：加味沙参麦冬汤 配方颗粒 液质联用 差异性成分 

分 类 号：R283[医药卫生—中药学]