无侧枝切花菊组培快繁体系的建立  

Establishment of Efficient and Rapid Propagation System for Tissue Culture of Non-branching Cut Chrysanthemum

作　　者：姚依岑 王子钰 丛天慈 高健洲[1] 袁存权 YAO Yicen;WANG Ziyu;CONG Tianci;GAO Jianzhou;YUAN Cunquan(Beijing Key Laboratory of Ornamental Plants Germplasm Innovation&Molecular Breeding/National Engineering Research Center for Floriculture/Beijing Laboratory of Urban and Rural Ecological Environment/Engineering Research Center of Landscape,Environment of Ministry of Education/Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education/School of Landscape Architecture,Beijing Forestry University,Beijing 100083)

机构地区：[1]花卉种质创新与分子育种北京市重点实验室,国家花卉工程技术研究中心,城乡生态环境北京实验室,园林环境教育部工程研究中心,林木花卉遗传育种教育部重点实验室,北京林业大学园林学院,北京100083

出　　处：《北方园艺》2025年第8期8-17,共10页Northern Horticulture

基　　金：雄安新区科技创新专项资助项目(2022XAGG0100);国家自然科学基金资助项目(32071827);中央高校基本科研业务费专项资金资助项目(QNTD202306)。

摘　　要：以无侧枝切花菊品种‘圣雪’为试材,以茎尖和单芽茎段2种材料为外植体,采用植物组织培养的方法,研究了不同外植体、不同浓度植物生长调节剂对切花菊生根和分化的影响,以期建立高效稳定的无侧枝切花菊组织培养再生体系,为解决我国市场中缺乏无侧枝切花菊种苗提供参考依据。结果表明:茎尖比单芽茎段更适合作为外植体,其中4~5 mm的茎尖效果比2~3 mm的茎尖好。在茎尖的初代培养中,MS+0 mg·L^(-1)6-BA+0 mg·L^(-1)NAA更适宜用来直接促进茎尖萌发,MS+2.0 mg·L^(-1)6-BA+0.3 mg·L^(-1)NAA与MS+1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1)NAA更适宜用来诱导丛生芽产生,MS+1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1)NAA和1/2MS+0.1 mg·L^(-1)NAA分别可以用作菊花的继代与生根培养基使用,增殖、生根效果均良好。最终建立了切花菊品种‘圣雪’高效稳定的组织培养再生体系。Taking the stem tips and single bud stem segments of the cut chrysanthemum variety‘Shengxue’as the test material,the effects of different materials and concentrations of plant growth regulators on the rooting and differentiation of cut chrysanthemum were studied by using plant tissue culture techniques,in order to establish a highly efficient and stable tissue culture and regeneration system for cut chrysanthemum without lateral branch,and provide reference for solve the lack of lateral branch cut chrysanthemum varieties in the Chinese market.The results showed that the stem tip was more suitable as an explant than a single bud stem segment,with a 4-5 mm shoot tip having better results than a 2-3 mm stem tip.In the initial culture of stem tips,MS+0 mg·L^(-1)6-BA+0 mg·L^(-1) NAA was the optimal medium.MS+1.0 mg·L^(-1)6-BA+0.1 mg·L^(-1) NAA and 1/2MS+0.1 mg·L^(-1) NAA could be used as subculture and rooting media for chrysanthemum,respectively,the effects of proliferation and rooting were better.Finally,an efficient and stable tissue culture regeneration system for‘Shengxue’has been developed.

关 键 词：无侧枝切花菊 组培再生 茎尖 单芽茎段 

分 类 号：S681.9[农业科学—观赏园艺]