林下参种子无菌萌发条件优化研究  

Study on Optimization of Aseptic Germination Conditions for Seeds of Understory Panax ginseng

作　　者：苗春泽[1] 陈翠红 任丽 陈翠楠 田年军 陈丽飞[3] MIAO Chunze;CHEN Cuihong;REN Li;CHEN Cuinan;TIAN Nianjun;CHEN Lifei(Baishan Academy of Forestry Science,Baishan,Jilin 134300;Baishan Wildlife Resources Supervision Center Station,Baishan,Jilin 134300;College of Horticulture,Jilin Agricultural University,Changchun,Jilin 130118)

机构地区：[1]白山市林业科学研究院,吉林白山134300 [2]白山市野生动植物资源监管中心站,吉林白山134300 [3]吉林农业大学园艺学院,吉林长春130118

出　　处：《北方园艺》2025年第8期82-88,共7页Northern Horticulture

基　　金：吉林省创新创业人才资助项目(2023RY18);白山市科技发展计划资助项目(20220003)。

摘　　要：以林下参种子为试材,采用组培快繁的方法,研究了不同消毒时间、基本培养基类型以及光照条件等因素对种子无菌萌发的影响,以期为林下参种子无菌萌发体系的建立及离体快繁技术的应用提供参考依据。结果表明:林下参种子最适消毒处理为使用消毒剂2%NaClO对种子表面进行消毒10 min,然后对种胚进行消毒7 s,该条件下种子消毒效果与萌发效果均为最佳;MS培养基为种子无菌萌发最适基本培养基,最适光照强度为1500 lx;添加单一激素6-BA或NAA可明显提高种子萌发率。整体萌发生长情况最佳的培养基为MS+6-BA 1.2 mg·L^(-1)+NAA 0.3 mg·L^(-1)。The seeds of understory Panax ginseng were used as the experimental materials,tissue culture and rapid propagation was adopted,the effects of different disinfection time,basic medium type and light conditions on aseptic germination of seeds were studied,in order to provide reference for the establishment of aseptic germination system and the application of the rapid propagation technology in vitro of understory Panax ginseng.The results showed that the most suitable disinfection treatment for the seeds of understory Panax ginseng was to disinfect the seed surface with 2%NaClO for 10 minutes,and then disinfect the embryo for 7 seconds,under these conditions,the seed disinfection and germination effects were both optimal.MS medium was the most suitable basic medium for aseptic germination of seeds,and the optimum light intensity was 1500 lx.The addition of a single hormone 6-BA or NAA could significantly improve the seed germination rate.The best medium for the overall germination and growth was MS+6-BA 1.2 mg·L^(-1)+NAA 0.3 mg·L^(-1).

关 键 词：林下参 种子 无菌萌发 培养基 

分 类 号：R284[医药卫生—中药学]