黄芪甲苷调节Notch信号通路对瘢痕疙瘩成纤维细胞增殖和迁移的影响  

The Impacts of Astragaloside IV on the Proliferation and Migration of Human Keloid Fibroblasts by Regulating the Notch Signaling Pathway

作　　者：蒋智永 席庆春 李旋[1] 桑鹏飞 JIANG Zhiyong;XI Qingchun;LI Xuan;SANG Pengfei(Department of Plastic and Reconstructive Burn Surgery,the Second People's Hospital of Hefei,(Hefei Hospital Affiliated to Anhui Medical University),Hefei 230000,China)

机构地区：[1]合肥市第二人民医院(安徽医科大学附属合肥医院)整形修复烧伤外科,合肥230000

出　　处：《中国细胞生物学学报》2025年第4期832-840,共9页Chinese Journal of Cell Biology

摘　　要：该文旨在探讨黄芪甲苷Ⅳ(简称黄氏甲苷)对人瘢痕疙瘩成纤维细胞(human keloid fibroblast,HKF)增殖和迁移的影响,分析其机制是否与Notch信号通路有关。将HKF分为NC组(正常培养)、L-黄芪甲苷组(25μmol/L黄芪甲苷)、M-黄芪甲苷组(50μmol/L黄芪甲苷)、H-黄芪甲苷组(100μmol/L黄芪甲苷)和H-黄芪甲苷+Notch信号通路激活剂Jagged1-Fc组(H-黄芪甲苷+Jagged1-Fc组,100μmol/L黄芪甲苷+0.5μg/mLJagged1-Fc)。使用CCK-8法、流式细胞术、划痕实验、Transwell法以及Western blot分别对HKF增殖、凋亡、迁移、侵袭以及PCNA、MMP-2、collagen-Ⅰ、collagen-Ⅲ、Notch1、Hes1蛋白表达水平进行检测。L-黄芪甲苷组、M-黄芪甲苷组、H-黄芪甲苷组较NC组细胞D值、划痕愈合率、侵袭细胞数以及PCNA、MMP-2、collagen-Ⅰ、collagen-Ⅲ、Notch1、Hes1蛋白表达水平降低,细胞凋亡率升高(P<0.05);与H-黄芪甲苷组比较,H-黄芪甲苷+Jagged1-Fc组D值、划痕愈合率、侵袭细胞数以及PCNA、MMP-2、collagen-Ⅰ、collagen-Ⅲ、Notch1、Hes1蛋白表达水平显著增加,细胞凋亡率显著降低(P<0.05)。黄芪甲苷可能通过抑制Notch信号通路,进而抑制HKF增殖和迁移。This study aims to investigate the impacts of astragaloside IV on the proliferation and migration of HKF(human keloid fibroblast),and to analyze whether its mechanism is related to the Notch signaling pathway.HKF was assigned into NC group(normal culture),L-astragaloside IV group(25μmol/L astragaloside IV),M-astragaloside IV group(50μmol/L astragaloside IV),H-astragaloside IV group(100μmol/L astragaloside IV),and H-astragaloside IV+Notch signaling pathway activator Jagged1-Fc group(100μmol/L astragaloside IV+0.5μg/mL Jagged1-Fc group).CCK-8 method,flow cytometry,scratch assay,Transwell method,and Western blot separately were used to detect the proliferation,apoptosis,migration,invasion,and the expression of PCNA,MMP-2,collagen-I,collagen-III,Notchl,and Hesl proteins in HKF.The D value of cells,scratch healing rate,number of invasive cells,expression of PCNA,MMP-2,collagen-I,collagen-III,Notch1,and Hesl proteins in L-astragaloside IV group,M-astragaloside IV group,and H-astragaloside IV group were lower than those in NC group,and the apoptosis rate was higher(P<0.05).The D value,scratch healing rate,number of invasive cells,expression of PCNA,MMP-2,collagen-I,collagen-III,Notchl,and Hesl proteins in H-astragaloside IV+Jagged1-Fc group were prominently higher than those in H-astragaloside IV group,and the cell apoptosis rate was prominently lower(P<0.05).Astragaloside IV may inhibit the proliferation and migration of HKF by suppressing the Notch signaling pathway.

关 键 词：黄芪甲苷 NOTCH信号通路 人瘢痕疙瘩成纤维细胞 增殖 迁移 

分 类 号：R285[医药卫生—中药学]