脐带间充质干细胞生物学特性鉴定及对博来霉素介导的急性肺损伤小鼠的修复效应研究  

Study on the biological characteristics of umbilical cord mesenchymal stem cells and their reparative effects on bleomycin-induced acute lung injury in mice

作　　者：文河保 叶文豪 邹孝芸 马彩云 刘淳博 李向臣[2] 刘长青 关伟军[2] WEN Hebao;YE Wenhao;ZOU Xiaoyun;MA Caiyun;LIU Chunbo;LI Xiangchen;LIU Changqing;GUAN Weijun(Anhui Engineering Research Center for Neural Regeneration Technology and Medical New Materials,Bengbu Medical University,Bengbu,Anhui,233000,P.R.China;Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,P.R.China)

机构地区：[1]蚌埠医科大学安徽省神经再生技术与医用新材料工程研究中心,安徽蚌埠233000 [2]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《中国呼吸与危重监护杂志》2025年第4期260-267,共8页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：国家自然科学基金面上项目(82371382);安徽省高校自然科学研究重点项目(KJ2021A0784、2022AH051434、2024AH051296);蚌埠医科大学组织移植安徽省重点实验室开放课题(AHTT2022B001);蚌埠医科大学国自然基金孵育项目(2023byfy007);蚌埠医科大学质量工程实验教学和教学实验室项目(2024syyj05);国家级大学生创新训练项目(202310367061);安徽省高等学校省级质量工程项目(2023jyxm0653)。

摘　　要：目的探究鸡脐带间充质干细胞(umbilical cord mesenchymal stem cells,UMSC)的多向分化潜能等生物学特征以及对博来霉素(bleomycin,BLM)介导的肺损伤小鼠的修复效应。方法向小鼠肺支气管注射BLM,构建小鼠急性肺损伤模型,通过尾静脉移植UMSC,病理学切片的观察、移植细胞在小鼠体内存活及分化、羟脯氨酸(Hydroxyproline,HYP)含量等检测指标,评估分析UMSC对小鼠肺损伤的修复效应。结果本研究成功分离的UMSCs,阳性表达特异性表面标记物CD29、CD44、CD90和CD166,而CD34与CD45的表达呈阴性,且诱导培养的UMSC可以向脂肪、成骨、软骨和肺泡上皮细胞分化;动物实验发现,经BLM处理的小鼠肺泡结构受损,肺泡内炎症细胞浸润、异常的胶原蛋白沉积,移植UMSC治疗后,小鼠肺部受损的面积和程度降低,肺组织HYP含量下降但高于对照组。结论UMSC在体外培养条件下可以持续扩增、保持其生物学特性,并具有定向迁移至受损部位、定向分化的能力,对BLM诱导的急性肺损伤小鼠有一定的修复效应。Objective To investigate the multi-directional differentiation potential and other biological characteristics of chicken umbilical cord mesenchymal stem cells(UMSC),as well as their reparative effects on bleomycin(BLM)-induced lung injury in mice.Methods An acute lung injury model in mice was established by injecting BLM into the bronchus.UMSC were then transplanted via the tail vein.The reparative effects of UMSC on lung injury were evaluated through pathological section observation,survival and differentiation of transplanted cells in mice,and detection of hydroxyproline(HYP)content,among other indicators.Results The UMSC successfully isolated in this study positively expressed specific surface markers CD29,CD44,CD90,and CD166,while the expression of CD34 and CD45 was negative.Induced UMSC could differentiate into adipocytes,osteocytes,chondrocytes,and alveolar epithelial cells.Animal experiments revealed that BLM-treated mice exhibited damaged alveolar structures,significant inflammatory cell infiltration,abnormal collagen deposition,and pulmonary fibrosis.However,after UMSC transplantation,the extent and severity of lung damage were reduced,and the HYP content in lung tissue decreased but remained higher than that of the control group.Conclusion UMSC can continuously proliferate and maintain their biological characteristics under in vitro culture conditions.They possess the ability to migrate to damaged sites and undergo directional differentiation,demonstrating a certain reparative effect on BLM-induced acute lung injury in mice.

关 键 词：脐带间充质干细胞 多分化潜能 移植治疗 肺损伤 修复效应 

分 类 号：R563[医药卫生—呼吸系统]