小麦新品种济麦379的抗旱机理初探  

Preliminary Study on Drought Resistance Mechanism of New Wheat Variety Jimai 379

作　　者：徐向敏 杨在东 崔德周[3] 黄琛 樊庆琦[3] 隋新霞[3] 陈世华[1] 楚秀生 李永波[3] XU Xiangmin;YANG Zaidong;CUI Dezhou;HUANG Chen;FAN Qingqi;SUI Xinxia;CHEN Shihua;CHU Xiusheng;LI Yongbo(School of Life Sciences,Yantai University,Yantai 264005,China;Shandong Luyan Agriculture Co.,Ltd,Jinan 250100,China;Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,China)

机构地区：[1]烟台大学生命科学学院,山东烟台264005 [2]山东鲁研农业良种有限公司,山东济南250100 [3]山东省农业科学院作物研究所,山东济南250100

出　　处：《烟台大学学报(自然科学与工程版)》2025年第2期202-207,共6页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：国家重点研发计划课题(2022YFD1200205,2022YFD1201704)。

摘　　要：为探究小麦新品种济麦379的抗旱机理,利用分子生物学技术结合田间试验,通过对济麦379和受体对照济麦06037进行干旱处理,分析其抗旱表型以及细胞自噬差异。结果表明:干旱胁迫下济麦379苗期持绿期比济麦06037长约3 d,而且其根长、芽长分别比济麦06037增加1.70 cm和2.16 cm;旱地试验结果显示:济麦379的小区产量显著高于济麦06037;免疫组织化学结果显示:在干旱胁迫的济麦379根部细胞中出现大量的自噬结构,而在济麦06037根部细胞中则较少;Western blot结果显示:干旱胁迫下济麦379中自噬相关蛋白ATG8、ATG8-PE的表达量更多。由此表明,济麦379的抗旱性与自噬能力强于济麦06037。推测细胞自噬参与了济麦379的抗旱调控过程,与济麦379的抗旱性呈正相关。本研究结果为进一步研究自噬参与小麦抗旱调控机理,以及从济麦379中挖掘优异抗旱基因提供一定理论基础。To explore the drought resistance mechanism of the new wheat variety Jimai 379,differences in drought-resistant phenotype and autophagy between Jimai 379 and the receptor Jimai 06037 were analyzed using molecular biology techniques and field trials.The results showed that the seedling green-holding period of Jimai 379 isabout 3 days longer,and its root length and bud length are 1.70 cm and 2.16 cm longer than those of Jimai 06037under drought stress.Results from dry field trials indicate that the yield of Jimai 379 is significantly higher than that of Jimai 06037.Immunohistochemical results reveal that numerous autophagy structures are present in the root cells of Jimai 379 under drought stress,whereas fewer are found in the root cells of Jimai 06037.Western blot results demonstrate that the expressions of autophagy-related proteins ATG8 and ATG8-PE in Jimai 379 are higher under drought stress.These findings suggest that the drought resistance and autophagy capability of Jimai 379 are stronger than those of Jimai 06037.It is hypothesized that autophagy plays a role in regulating the drought resistance of Jimai 379,which is positively correlated with its drought resistance.The outcomes of this study will provide a theoretical foundation for further research into the mechanism by which autophagy regulatesdrought resistance in wheat and for identifying excellent drought resistance genes in Jimai 379.

关 键 词：小麦 抗旱 自噬 ATG8 调控 

分 类 号：S512[农业科学—作物学]