Promising protective treatment potential of endophytic bacterium Rhizobium aegyptiacum for ulcerative colitis in rats  

作　　者：Engy ELEKHNAWY Duaa ELIWA Sebaey MAHGOUB Sameh MAGDELDIN Ehssan MOGLAD Sarah IBRAHIM Asmaa Ramadan AZZAM Rehab AHMED Walaa A.NEGM 

机构地区：[1]Pharmaceutical Microbiology Department,Faculty of Pharmacy,Tanta University,Tanta 31527,Egypt [2]Department of Pharmacognosy,Faculty of Pharmacy,Tanta University,Tanta 31527,Egypt [3]Proteomics and Metabolomics Research Program,Department of Basic Research,Children's Cancer Hospital 57357,Cairo I1441,Egypt [4]Department of Physiology,Faculty of Veterinary Medicine,Suez Canal University,Ismailia 41522,Egypt [5]Department of Pharmaceutics,College of Pharmacy,Prince Sattam bin Abdulaziz University,Po.Box 173,Alkharj 11942,Saudi Arabia [6]Human Anatomy and Embryology Department,Faculty of Medicine,Tanta University,Tanta 31527,Egypt [7]Division of Microbiology,Immunology and Biotechnology,Department of Natural Products and Alternative Medicine,Faculty of Pharmacy,University of Tabuk,Tabuk47713,Saudi Arabia

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2025年第3期286-301,共16页浙江大学学报(英文版)B辑(生物医学与生物技术)

基　　金：funding provided by The Science,Technology&Innovation Funding Authority(STDF)in cooperation with The Egyptian Knowledge Bank(EKB).

摘　　要：Ulcerative colitis(UC)is an inflammatory condition of the intestine,resulting from an increase in oxidative stress and pro-inflammatory mediators.In this study,the extract of endophytic bacterium Rhizobium aegyptiacum was prepared for the first time using liquid chromatography-mass spectrometry(LC-MS).In addition,also for the first time,the protective potential of R.aegyptiacum was revealed using an in vivo rat model of UC.The animals were grouped into four categories:normal control(groupⅠ),R.aegyptiacum(groupⅡ),acetic acid(AA)-induced UC(groupⅢ),and R.aegyptiacum-treated AA-induced UC(groupⅣ).In groupⅣ,R.aegyptiacum was administered at 0.2 mg/kg daily for one week before and two weeks after the induction of UC.After sacrificing the rats on the last day of the experiment,colon tissues were collected and subjected to histological,immunohistochemical,and biochemical investigations.There was a remarkable improvement in the histological findings of the colon tissues in groupⅣ,as revealed by hematoxylin and eosin(H&E)staining,Masson's trichrome staining,and periodic acid-Schiff(PAS)staining.Normal mucosal surfaces covered with a straight,intact,and thin brush border were revealed.Goblet cells appeared magenta in color,and there was a significant decrease in the distribution of collagen fibers in the mucosa and submucosal connective tissues.All these findings were comparable to the respective characteristics of the control group.Regarding cyclooxygenase-2(COX-2)immunostaining,a weak immune reaction was shown in most cells.Moreover,the colon tissues were examined using a scanning electron microscope,which confirmed the results of histological assessment.A regular polygonal unit pattern was seen with crypt orifices of different sizes and numerous goblet cells.Furthermore,the levels of catalase(CAT),myeloperoxidase(MPO),nitric oxide(NO),interleukin-6(IL-6),and interlukin-1β(IL-1β)were determined in the colonic tissues of the different groups using colorimetric assay and enzyme-linked immunosorbent assay(ELIS

关 键 词：Inflammatory bowel syndrome Liquid chromatography-tandem mass spectrometry(LC-MS/MS) Scanning electron microscopy(SEM) HISTOLOGY Immunohistochemistry Reverse transcription-quantitative polymerase chain reaction(RT-qPCR) 

分 类 号：R285.5[医药卫生—中药学]