基于ITS2序列的香薷属药用植物鉴别  

Identification of medicinal plants of Elsholtzia based on ITS2 sequence

作　　者：李晓晗 熊超 徐燃[1] LI Xiaohan;XIONG Chao;XU Ran(School of Life Science and Technology,Wuhan Polytechnic University,Wuhan 430023,China)

机构地区：[1]武汉轻工大学生命科学与技术学院,武汉430023

出　　处：《武汉轻工大学学报》2025年第2期50-55,共6页Journal of Wuhan Polytechnic University

基　　金：2024年湖北省自然科学基金(编号:2024AFB1020).

摘　　要：通过DNA条形码中的内转录间隔区2(Internal eranscribed spacer,ITS2)序列鉴定香薷(Elsholtzia ciliata)及其同属近缘植物种,建立鉴定香薷属药用植物的有效方法。采集14份不同产地香薷属药用植物,并对其ITS2序列扩增和测序,在GenBank上下载同属物种的21条ITS2序列,共得到35条ITS2序列。基于Kimura-2-parameter(K2P)模型计算种内种间遗传距离,邻接法(Neighbor-joining,NJ)构建系统发育树,通过ITS2数据库预测ITS2序列的二级结构。结果表明香薷属药用植物的ITS2序列长度为219~233 bp;香薷属药用植物种间遗传距离大于种内遗传距离,存在明显的barcoding gap;NJ系统发育树显示,不同物种得到良好的区分;ITS2二级结构中香薷与其他物种在4个螺旋区中的臂长,内环大小和数量存在明显差异。本研究基于ITS2序列的DNA条形码技术能成功鉴定香薷及其同属近缘植物种。The internal transcribed spacer 2(ITS2)sequences in DNA barcoding were employed in DNA barcoding to identify Elsholtzia ciliata and its closely related species,as well as to develop a reliable method for distinguishing E.ciliata.ITS2 sequences were amplified and sequenced from 14 Elsholtzia plants of diverse origins,and 21 ITS2 sequences from the same genus were retrieved from GenBank.In total,35 ITS2 sequences were obtained.The Kimura-2-Parameter(K2P)model was employed to analyze intraspecific and interspecific genetic distance,the Neighbor-joining(NJ)method was used to construct a phylogenetic tree,and ITS2 database was utilized to predict the secondary structure of ITS2.The results indicated that ITS2 sequence length in Elsholtzia ranged from 219 to 233 bp.The interspecific genetic distance of Elsholtzia was larger than the intraspecific genetic distance,with a clear barcoding gap observed between the species.The NJ phylogenetic tree demonstrated clear differentiation among species.Significant differences in arm length,inner ring size and the number of inner ring were observed between E.ciliata and other species within four spiral regions of the ITS2 secondary structure.This study demonstrates that DNA barcoding technology based on ITS2 sequence can effectively identify E.ciliata and its related species.

关 键 词：香薷 香薷属 DNA条形码 ITS2序列 分子鉴定 

分 类 号：Q9[生物学]