高葡萄糖诱导对293T细胞类二十烷酸代谢的影响  

Effect of high glucose induction on eicosanoid metabolism in 293T Cells

作　　者：曹洋 柏聪颖 王文倩 李浩 杜佳欣 郑柏诚 李捷 魏利莎 CAO Yang;BAI Cong-ying;WANG Wen-qian;LI Hao;DU Jia-xin;ZHENG Bai-cheng;LI Jie;WEI Li-sha(School of Marine and Biological Engineering,Yancheng Teachers University,Yancheng 224000,Jiangsu,China)

机构地区：[1]盐城师范学院海洋与生物工程学院,江苏盐城224000

出　　处：《湖北农业科学》2025年第4期171-175,共5页Hubei Agricultural Sciences

基　　金：国家自然科学青年基金项目(82200976)。

摘　　要：采用高葡萄糖(50 mmol/L)诱导293T细胞,通过CCK-8试剂盒检测细胞在1~4 d的增殖情况,利用荧光实时定量PCR技术分析细胞中类二十烷酸代谢相关基因及炎症相关基因的mRNA表达水平。结果表明,与正常葡萄糖(25 mmol/L)相比,高葡萄糖刺激后,293T细胞内类二十烷酸代谢相关基因的mRNA表达水平未发生显著变化,仅COX1基因在第5代时出现短暂下调,随后恢复至正常水平。高葡萄糖刺激还导致293T细胞内炎症因子IL-1β的mRNA水平在第11代时显著升高,表明长期高葡萄糖刺激可能诱发293T细胞产生轻度炎症反应。293T cells were induced with high glucose(50 mmol/L).The cell proliferation from days 1 to 4 was detected using a CCK-8 kit,and the mRNA expression levels of eicosanoid metabolism-related genes and inflammation-related genes were analyzed by realtime fluorescent quantitative PCR.The results showed that,compared with normal glucose(25 mmol/L),the mRNA expression levels of eicosanoid metabolism-related genes in 293T cells did not change significantly after high glucose stimulation.Only the COX1 gene exhibited transient downregulation at the 5th passage,which subsequently returned to normal levels.High glucose stimulation also significantly increased the mRNA level of the inflammatory factor IL-1βin 293T cells at the 11th passage,suggesting that long-term high glucose stimulation might induce mild inflammatory responses in 293T cells.

关 键 词：高葡萄糖 诱导 类二十烷酸代谢 293T细胞 

分 类 号：R589[医药卫生—内分泌]