bAD-MSCs水凝胶对奶牛子宫内膜炎的疗效分析  

Effect Analysis of bAD-MSCs Hydrogel on Endometritis in Dairy Cows

作　　者：韩双印 付春宇 王峥钰 黄子轩 于泳 邢晓娅 鲁文赓[1] Han Shuangyin;Fu Chunyu;Wang Zhengyu;Huang Zixuan;Yu Yong;Xing Xiaoya;Lu Wengeng(College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319;College of Life Science and Technology,Heilongjiang Bayi Agricultural University)

机构地区：[1]黑龙江八一农垦大学动物科技学院,大庆163319 [2]黑龙江八一农垦大学生命科学技术学院

出　　处：《黑龙江八一农垦大学学报》2025年第2期15-22,共8页journal of heilongjiang bayi agricultural university

基　　金：黑龙江省“揭榜挂帅”科技攻关项目(2023ZXJ02B03)。

摘　　要：奶牛子宫内膜炎是导致奶牛养殖业经济效益降低的常见多发病,及时有效地防治奶牛子宫内膜炎具有重大意义。将奶牛脂肪间充质干细胞(bovine adipose-derived mesenchymal stem cells,bAD-MSCs)经细胞超声破碎浓缩后与卡波姆水凝胶溶液融合,制备成bAD-MSCs水凝胶。选取15头患子宫内膜炎的奶牛,分别向子宫角内注入卡波姆水凝胶溶液(n=5,阴性对照组)、抗生素-利福昔明宫内注入剂(n=5,阳性对照组)和bAD-MSCs水凝胶(n=5,试验/治疗组),而后通过超声波检测法、细胞学检查法和ELISA法检测其体内炎症反应及血清中炎症因子的表达情况,分析bAD-MSCs水凝胶对奶牛子宫内膜炎的治疗效果。注入治疗7 d后,与对照组相比,超声波图像显示,bAD-MSCs水凝胶治疗组的子宫内回声强度显著减弱(P<0.05);子宫颈外口分泌物涂片细胞学检查发现,bAD-MSCs水凝胶治疗组的炎性细胞数量减少,中性粒细胞(PMN)所占比例显著下降(P<0.05);b AD-MSCs水凝胶治疗组的血清中TNF-α、IL-1β、IL-6和IL-10炎症因子表达显著降低(P<0.05)。结果表明,bAD-MSCs水凝胶对奶牛子宫内膜炎的防治具有良好的效果。Endometritis in dairy cows is a common and frequent disease that leads to the reduction of economic benefits of dairy farming,and it is of great significance to prevent and treat endometritis in dairy cows in a timely and effective manner.Bovine adipose-derived mesenchymal stem cells(bAD-MSCs)were fused with hydrogel solution after ultrasonic disruption and concentration to prepare bAD-MSCs hydrogels.Fifteen cows with endometritis were selected and infused hydrogel solution(n=5,negative control group),rifaximin Intrauterine Infusion(n=5,positive control group)and bAD-MSCs hydrogel(n=5,test/treatment group)into the uterine horn,respectively.Then the inflammatory response and the expression of inflammatory factors in serum was detected by ultrasound,cytology and ELISA,and the therapeutic effect of bAD-MSCs hydrogel on endometritis in dairy cows was analyzed.Seven days after infusion treatment,compared with the control group,ultrasound images showed intrauterine echo intensity in the bAD-MSCs hydrogel treatment group was significantly reduced(P<0.05),the cytological examination of Smears from swab samples of the external os of the cervix showed that the number of inflammatory cells and the proportion of neutrophil(PMN)in bAD-MSCs hydrogel treatment group decreased significantly(P<0.05),the expressions of TNF-α、IL-1β、IL-6 and IL-10 inflammatory factors in serum of bAD-MSCs hydrogel treatment group were significantly decreased(P<0.05)were significantly reduced by ELISA(P<0.05).The results indicated that bAD-MSCs hydrogel had good effect on the prevention and treatment of endometritis in dairy cows.

关 键 词：牛脂肪间充质干细胞 水凝胶 奶牛子宫内膜炎 炎症 

分 类 号：S857.21[农业科学—临床兽医学]