云南省2023年国家级登革热监测点登革热病原监测分析  

Analysis on etiological surveillance of dengue fever cases at national dengue monitoring sites in Yunnan Province in 2023

作　　者：郭晓芳[1] 容艺函宇 黄兴云 李香银 陈黎媛 王雪菲 唐烨榕 GUO Xiaofang;RONG Yihanyu;HUANG Xingyun;LI Xiangyin;CHEN Liyuan;WANG Xuefei;TANG Yerong(Yunnan Provincial Key Laboratory of Vector-Borne Infectious Diseases Control and Research,Yunnan Institute of Parasitic Diseases Innovative Team of Key Techniques for Vector Borne Disease Control and Prevention,Yunnan Institute of Parasitic Diseases,Pu'er,Yunnan 665000,China;Menghai County Center for Disease Control and Prevention,Menghai,Yunnan 666200,China;Longchuan County Center for Disease Control and Prevention,Longchuan,Yunnan 678700,China;Hekou County Center for Disease Control and Prevention,Hekou,Yunnan 661300,China)

机构地区：[1]云南省寄生虫病防治所,云南省虫媒传染病防控研究重点实验室,云南省虫媒传染病防控关键技术创新团队,云南普洱665000 [2]勐海县疾病预防控制中心,云南勐海666200 [3]陇川县疾病预防控制中心,云南陇川678700 [4]河口县疾病预防控制中心,云南河口661300

出　　处：《中国热带医学》2025年第2期141-148,共8页China Tropical Medicine

基　　金：国家自然科学基金项目(No.81560548)。

摘　　要：目的 了解2023年云南省登革热病原种类及来源,为登革热防控提供科学依据。方法 对2023年云南省登革热国家级监测点(陇川县、勐海县、河口县)的登革热病例血清进行登革病毒(dengue virus, DENV)核酸检测及病毒分离培养,对阳性血清和登革病毒分离株进行登革病毒E基因测序,采用分子生物学软件进行系统进化分析。结果 2023年3个监测点共报告1 006例登革热病例,其中输入病例838例,云南其他县市带入病例161例,本地病例7例。从371份血清中检测到351份DENV阳性血清,其中305份为DENV-1阳性(包括缅甸输入174例、老挝输入1例、越南输入1例、景洪带入99例、瑞丽带入26例、本地病例4例)、43份为DENV-2阳性(缅甸输入8例、景洪带入6例、瑞丽带入26例、本地病例3例),1份为DENV-3阳性(缅甸输入),1份为DENV-4阳性(瑞丽带入),1份未分型。共分离到23株DENV,其中9株DENV-1、12株DENV-2、1株DENV-3、1株DENV-4。3个监测点的DENV-1均属于基因Ⅰ型,但位于不同的进化分支。勐海县和陇川县的DENV-2分别属于Cosmopolitan基因型和AsianⅠ基因型。陇川县的DENV-3属于基因Ⅰ型。陇川县的DENV-4属于基因Ⅰ型。结论 在登革热流行季节,云南边境地区存在境外和境内双重登革热病例输入压力。输入或带入共DENV-1~DENV-4型5个基因型DENV,发生了因输入或带入引起的本地疫情,输入和本地的DENV来源于东南亚国家并有地域分布差异的特点。Objective To understand the serotypes and sources of dengue virus(DENV)in Yunnan Province in 2023,providing a scientific basis for dengue fever prevention and control.Methods DENV nucleic acid testing and virus isolation were performed on the serum samples of dengue fever cases diagnosed at three national monitoring sites in Yunnan Province(Longchuan County,Menghai County,Hekou County)in 2023.Dengue virus envelope(E)gene sequencing was performed on positive serum samples and dengue virus isolates,and phylogenetic analysis was conducted using molecular biology software.Results A total of 1006 dengue fever cases were reported at three monitoring sites in 2023,including 838 imported cases,161 cases imported from other counties and cities in Yunnan,and 7 autochthonous cases.Among 371 serum samples,351 were found DENV-positive,including 305 DENV-1 positive samples(174 imported from Myanmar,1 imported from Laos,1 imported from Vietnam,99 imported from Jinghong,26 imported from Ruili,and 4 local cases),43 DENV-2 positive samples(8 imported from Myanmar,6 imported from Jinghong,26 imported from Ruili,and 3 local cases),1 DENV-3 positive sample(imported from Myanmar),1 DENV-4 positive sample(introduced from Ruili),and 1 untyped case.A total of 23 DENV strains were isolated,including 9 strains of DENV-1,12 strains of DENV-2,1 strain of DENV-3,and 1 strain of DENV-4.All DENV-1 strains from three monitoring sites belong to genotype I but were located on different evolutionary branches.The DENV-2 strains from Menghai County and Longchuan County belonged to the Cosmopolitan genotype and Asian I genotype,respectively.The DENV-3 strain from Longchuan County belonged to genotype I,and the DENV-4 strain from Longchuan County belonged to genotype I.Conclusions During the dengue fever epidemic season,the border regions of Yunnan Province face dual pressures from both international and domestic imported dengue cases.Imported or introduced cases carried DENVs of serotypes 1-4(with five genotypes in total),leading to local outbreaks ca

关 键 词：登革热 登革病毒 监测 云南省 

分 类 号：R373.33[医药卫生—病原生物学]