腹腔多发性细粒棘球蚴病1例及其病原分子生物学鉴定  

A case of abdominal multiple cystic echinococcosis and its pathogenic molecular biology identification

作　　者：陈亿雄[1] 王梓琪 刘金凤 王超 张晟 任萌 陈清凉[1] 高世同[2] CHEN Yixiong;WANG Ziqi;LIU Jinfeng;WANG Chao;ZHANG Sheng;REN meng;CHEN Qingliang;GAO Shitong(Baoan District Center for Disease Control and Prevention,Shenzhen,Guangdong 518101,Guangdong,China;Shenzhen Center for Disease Control and Prevention,Shenzhen,Guangdong 518055,China)

机构地区：[1]深圳市宝安区疾病预防控制中心,广东深圳518101 [2]深圳市疾病预防控制中心,广东深圳518055

出　　处：《中国热带医学》2025年第2期217-221,共5页China Tropical Medicine

基　　金：深圳市医学重点学科传染病防控项目(No.SZXK064);深圳市宝安区科技创新局资助项目(No.2023JD148)。

摘　　要：目的 了解广东省深圳市1例腹腔多发性细粒棘球蚴病病例的临床特点,并进行病原分子生物学鉴定,为棘球蚴病防治提供依据。方法 收集2024年1月深圳市1例棘球蚴病病例的临床和流行病学资料;对病例手术摘除的包囊组织进行病理切片,染色后镜检;提取囊组织样品核酸,采用聚合酶链反应(PCR)扩增线粒体NADH脱氢酶亚基1基因(NADH dehydrogenase subunit 1, ND1)、线粒体细胞色素C氧化酶1基因(mitochondria cytochrome C oxidase subunit 1, Cox1)基因序列并进行测序,使用Mega X、BLAST等软件进行序列比对及系统进化树分析。结果 患者男,29岁,西藏自治区那曲市居民,有家犬接触史及“肝棘球蚴病”病史。影像学提示腹腔多发性占位,考虑棘球蚴病复发;手术摘除腹腔不同部位囊性病变组织12块,囊组织病理切片可见棘球蚴的角质层、生发层和原头蚴。细粒棘球蚴特异性基因ND1和Cox1 PCR扩增阳性,扩增片段大小分别约为510 bp与285 bp。序列比对及系统进化树分析结果显示本研究序列与GenBank数据库中细粒棘球蚴G1型ND1基因(青海JX217890.1株),Cox1基因(西藏MH050610.1株)的同源性为99%,在系统进化树中与西藏、青海及新疆等地区细粒棘球蚴流行株亲缘关系接近,处于同一进化分支(G1型)。结论 患者为1例腹腔多发性细粒棘球蚴病输入病例,基因分型为G1型,感染来源于西藏可能性大。非流行区应提升对本病的监测和管理水平,加强对流行区输入的牲畜、农副产品的检验检疫,防止棘球蚴病疫情扩散。Objective To identify the clinical characteristics of a case of abdominal multiple cystic echinococcosis in Shenzhen City,Guangdong Province,and to characterize the molecular biology of pathogen,in order to provide a basis for control and prevention of echinococcosis.Methods Clinical and epidemiological data of 1 case of echinococcosis in Shenzhen City in January 2024 were collected.The pathological sections of the cyst tissue removed by surgery were examined by microscopy after staining.Nucleic acids were extracted from cyst tissue samples,and the ND1 and Cox1 gene sequences were amplified and sequenced by PCR.Sequence comparison and phylogenetic tree analysis were performed using Mega X,BLAST and other software.Results The patient,male,29 years old,a resident of Nyerong County,Nagqu City,Xizang Autonomous Region,had a history of exposure to domestic dogs and hepatic echinococcosis.Imaging showed abdominal multiple placeholder,consider echinococcosis recurrence;12 pieces of cystic lesions from different parts of the abdominal cavity were surgically removed,and the pathological sections of cystic tissues showed cuticle layer,germinal layer,protoscolex of echinococcosis.PCR amplification of the specific genes ND1 and Cox1 were positive,and the amplified fragments were about 510 bp and 285 bp,respectively.Sequence comparison and phylogenetic tree analysis results showed that the homology between the sequences in this study and the ND1 gene of Echinococcus granulosus type G1(JX217890.1,Qinghai),and Cox1 gene(MH050610.1,Xizang)in the GenBank database was 99%.It is closely related to the epidemic strains of Echinococcus granulosus in Xizang,Qinghai and Xinjiang in the phylogenetic tree,and is in the same evolutionary branch(type G1).Conclusion The patient was an imported case of abdominal multiple cystic echinococcosis,and the genotype was G1,with a high probability that the infection originated in Xizang.It is recommended to enhance the surveillance and management of echinococcosis in non-endemic areas,and to strengt

关 键 词：细粒棘球蚴病 基因型 系统进化树分析 Cox1基因 ND1基因 

分 类 号：R532[医药卫生—内科学]