基于基因组重测序开发中华包革耳(金耳菌)菌株的鉴别标记  

Identification markers for Naematelia sinensis strains based on genome resequencing

作　　者：沈真辉 杨林雷 曹瑶 罗祥英 杨爱霞 陆青青 子灵山 李荣春 SHEN Zhenhui;YANG Linlei;CAO Yao;LUO Xiangying;YANG Aixia;LU Qingqing;ZI Lingshan;LI Rongchun(Yunnan Provincial(Rare Edible Mushroom)Enterprise Engineering Technology Centre,Yunnan Junshijie Biotechnology Co.Ltd.,Kunming 650200,Yunnan,China;Yunnan Province ZHANG Jingsong Expert Workstation,Yunnan(Kunming)ZHANG Jingsong Medicinal Fungus Expert Workstation,Kunming 650200,Yunnan,China)

机构地区：[1]云南菌视界生物科技有限公司云南省级(珍稀食用菌)企业工程技术中心,云南昆明650200 [2]云南省张劲松专家工作站古云南(昆明)张劲松药用真菌专家工作站,云南昆明650200

出　　处：《菌物学报》2025年第4期44-58,共15页Mycosystema

基　　金：云南省张劲松专家工作站(202305AF150084);云南昆明张劲松药用真菌专家工作站(YSZJGZZ-2022043)。

摘　　要：以中华包革耳(俗称金耳菌)单孢菌株NX-20基因组为参考基因组,以4个不同交配型单孢菌株D13、D25、D40和D47基因组重测序数据为研究对象,利用生物信息学软件鉴定出大量的SNP和InDel变异位点并分析其特征。使用primer5.0软件设计SNP/InDel标记引物,筛选存在多态性差异的8对SNP和6对InDel标记引物对我国金耳栽培产业中广泛使用的14个中华包革耳菌株进行遗传多样性分析。SNP标记分析结果显示,J2F1菌株在8对SNP引物中均形成2种基因型,其他菌株仅有一种基因型。6对InDel标记引物分析结果显示,J2F1菌株在第1、2及第5对引物中能扩增出不同于其他菌株的条带,大部分菌株能扩增出一致的条带(如YL、YR、JB、LL及SC等菌株),显示出同种异名现象。本研究结果对我国中华包革耳栽培种质资源的遗传多样性有了比较明确的认识,所获得的SNP和InDel标记可进一步用于中华包革耳遗传分析,为中华包革耳遗传多样性、种质资源鉴定和保护及分子辅助育种等研究助力。Using the genome of Naematelia sinensis monospore strain NX-20 as the reference,resequencing data from four monospore strains(D13,D25,D40,and D47)with differentmating types was analyzed by using bioinformatics software to identify SNP and InDelpositions.Primer5.0 software was used to design SNP/InDel marker primers,resulting in 8pairs of SNP and 6 pairs of InDel markers with polymorphic differences for genetic diversityanalysis of 14 widely used N.sinensis strains in the cultivation industry.Results of SNPmarker analysis revealed that the J2F1 strain exhibited two genotypes in eight pairs of SNPprimers,while other strains showed only one genotype.The analysis results of 6 pair InDelmarkers showed that the J2F1 strain displayed unique band patterns in the 1st,2nd,and 5thpairs of primers compared to other strains,while most strains(such as YL,YR,JB,LL,andSC)exhibited consistent band patterns,indicating the phenomenon of synonyms.Thesefindings enhance our understanding of the genetic diversity of N.sinensis cultivated germplasmresources,and the identified SNP and InDel molecular markers can serve as valuable tools forgenetic analysis,germplasm resource identification,protection and molecular-assisted breedingin N.sinensis research.

关 键 词：中华包革耳 单孢 重测序 插入缺失标记 单核苷酸多型性标记 

分 类 号：S646[农业科学—蔬菜学]