铜离子对黑木耳漆酶活性及其基因转录水平的影响  

Effects of copper ions on the laccase activities and gene transcription level of Auricularia heimuer

作　　者：米昊雨 耿楠楠 杜鹏禹 刘增才 邹莉[1] MI Haoyu;GENG Nannan;DU Pengyu;LIU Zengcai;ZOU Li(School of Forestry,Northeast Forestry University,Harbin 150040,Heilongjiang,China)

机构地区：[1]东北林业大学林学院,黑龙江哈尔滨150040

出　　处：《菌物学报》2025年第4期68-80,共13页Mycosystema

基　　金：山东省重点研发计划(2024LZGCQY018)。

摘　　要：以黑木耳“黑厚圆”为研究对象,探究铜离子对黑木耳漆酶活性及漆酶基因转录表达的影响。结果表明,铜离子浓度在100–700μmol/L范围内,黑木耳的胞外漆酶活性随着浓度的增加显著提高;同时,铜离子浓度显著影响漆酶基因的转录水平,其中AhLac1、AhLac3、AhLac6基因转录水平随铜离子浓度增加显著上调,AhLac2、AhLac4和AhLac5基因表达量在500μmol/L时达到最高值,分别上调了3.38、1.29和1.59倍。不同诱导时间下,AhLac1、AhLac3和AhLac6基因转录水平随诱导时间延长呈现不同程度的上调趋势。进一步的相关性分析显示,漆酶活性与AhLac1、AhLac3、AhLac6基因的转录水平之间存在正相关关系。研究结果为提高黑木耳产量及漆酶基因的分子调控机制研究提供科学依据。The effect of copper ions on laccase activity and transcriptional expression of laccase genes in Auricularia heimuer“Hei Hou Yuan”is investigated.The results show that as the copper ion concentration increases from 100 to 700μmol/L,extracellular laccase activity in A.heimuer increases significantly.Copper ion concentration strongly influences the transcriptional levels of laccase genes.Specifically,the transcription levels of AhLac1,AhLac3,and AhLac6 are significantly upregulated as the copper ion concentration rises.The expression levels of AhLac2,AhLac4,and AhLac5 peak at 500μmol/L,and increase by 3.38-,1.29-,and 1.59-fold,respectively.Under different induction times,the transcription levels of AhLac1,AhLac3,and AhLac6 show varied degrees of upregulation with the extension of induction period.Correlation analysis further reveals a positive relationship between laccase activity and the transcriptional levels of AhLac1,AhLac3,and AhLac6.This study provides a scientific basis for improving the yield of A.heimuer and investigating the molecular regulatory mechanisms underlying laccase gene expression.

关 键 词：黑木耳 铜离子 漆酶活性 转录表达 

分 类 号：S646.6[农业科学—蔬菜学]