蛹虫草水提物及其对HaCaT细胞氧化损伤的保护作用  

Cordyceps militaris aqueous extract and its protective effects against oxidative damage in HaCaT cells

作　　者：孙彦庆 张京良 朱宗敏 江晓路 尚丽丽 宗雯雯 SUN Yanqing;ZHANG Jingliang;ZHU Zongmin;JIANG Xiaolu;SHANG Lili;ZONG Wenwen(Qingdao Drenergy Biotechnology CO.,LTD.,Qingdao 266100,Shandong,China;Qingdao Marine Biomedical Research Institute,Qingdao 266100,Shandong,China)

机构地区：[1]青岛黛优佳生物科技有限公司,山东青岛266100 [2]青岛海洋生物医药研究院,山东青岛266100

出　　处：《菌物学报》2025年第4期123-135,共13页Mycosystema

摘　　要：本研究对蛹虫草水提物(CME)的主要活性成分及抗氧化活性进行分析,并建立H_(2)O_(2)诱导的HaCaT氧化损伤模型,探究其对皮肤细胞氧化损伤的保护作用。结果显示,CME中富含多糖、多酚、腺苷、虫草素、N^(6)-(2-羟乙基)腺苷等活性物质,且能够有效清除DPPH、ABTS和·OH自由基,IC_(50)分别为0.63、2.97和3.24 mg/mL,提示其具有良好的抗氧化能力;在H_(2)O_(2)诱导的HaCaT细胞中,CME随浓度递增而显著地提高细胞活力,其中50μg/mL CME预处理可使HaCaT细胞存活率由55.39%提高到83.90%;分析其作用机制,CME可能通过上调细胞内SOD、GSH-Px和CAT等抗氧化酶活力,减少细胞内丙二醛(MDA)含量,极显著地降低细胞内活性氧(ROS)水平(P<0.01),从而平衡氧化和抗氧化体系降低氧化损害。因此,CME具有保护皮肤细胞氧化损伤的作用,提示其具有成为天然抗氧化剂应用于化妆品等领域的潜力。The main active components and antioxidant activities of Cordyceps militaris aqueous extract(CME)were analyzed,and protective effects of the extract on skin cell oxidative damage in HaCaT cell model induced by hydrogen peroxide were evaluated.The results showed that CME was rich in polysaccharides,polyphenols,adenosine,cordycepin,N^(6)-(2-hydroxyethyl)adenosine and other active substances,and could scavenge DPPH free radical,ABTS free radical and·OH free radical,with IC_(50) values of 0.63,2.97 and 3.24 mg/mL,respectively,indicating the CME possessed effective antioxidant activity.The CME could improve HaCaT cell survival rate through concentration-dependent manner,and the cell viability was increased from 55.39%to 83.90%at the concentration of 50μg/mL.CME could reduce the intracellular malondialdehyde(MDA)content and significantly decrease the intracellular reactive oxygen species(ROS)level by up-regulating the activity of antioxidant enzymes such as SOD,GSH-Px and CAT(P<0.01),thereby balancing oxidation and antioxidant systems and reducing oxidative damage.This study comes to the conclusion that CME can protect skin cell avoiding oxidative damage of hydrogen peroxide,suggesting that it has the potential to be used as a natural antioxidant in cosmetics and other fields.

关 键 词：蛹虫草水取物 过氧化氢 氧化损伤 抗氧化 

分 类 号：R285[医药卫生—中药学]