Bacillus atrophaeus DX-9 biocontrol against potato common scab involves significant changes in the soil microbiome and metabolome  

作　　者：Jingjing Cao Yue Ma Jing Fu Zhiqin Wang Yonglong Zhao Naiqin Zhong Pan Zhao 

机构地区：[1]State Key Laboratory of Plant Genomics,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China [2]Engineering Laboratory for Advanced Microbial Technology of Agriculture,Chinese Academy of Sciences,Beijing 100101,China [3]School of Agriculture,Ningxia University,Yinchuan 750000,China [4]Qi Biodesign,Beijing 100101,China [5]Key Laboratory of Potato Industry Integration and Development Enterprises in Inner Mongolia Autonomous Region,Hulunbuir 021000,China

出　　处：《aBIOTECH》2025年第1期33-49,共17页生物技术通报(英文版)

基　　金：supported by the Inner Mongolia Autonomous Region Key Technology Tackling Programmed(Grant number:2021GG0300);by Hebei Science and Technology Major Project(22287501Z);by the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant number:XDA28030202-3,XDA24020104).

摘　　要：Potato common scab(CS)is a worldwide disease,caused by Streptomyces spp.,and its presence reduces the market value of potatoes.A nontoxic and potentially effective approach in many control strategies is the use of antagonistic microbes as biocontrol agents.In this study,Bacillus atrophaeus DX-9 was isolated and assessed for its ability to protect against CS.Through integrated metagenomic and metabolomic analyses,changes in the soil microbial community structure and soil properties were analyzed to understand the effects of Bacillus atrophaeus DX-9 on CS.These studies revealed that DX-9 inoculation could significantly decrease CS disease rate,disease index,and the number of CS pathogens,along with an increase in soil N and P content.Our metagenomic assays identified 102 phyla and 1154 genera,and DX-9 inoculation increased the relative abundances of the phyla Pseudomonadota,Chloroflexota and Gemmatimonadota.Additionally,an increase in the relative abundance of genera,such as Bradyrhizobium,Agrobacterium,and Nitrobacter,were significantly and positively correlated with soil N and P.Metabolomic analysis revealed that DX-9 inoculation significantly increased the soil levels of phytolaccoside A,7,8-dihydropteroic acid,novobiocin,and azafrin.These compounds were enriched in microbe pathway metabolites,including xenobiotic biodegradation and metabolism,biosynthesis of other secondary metabolites,and metabolism of cofactors and vitamins.In summary,the use of Bacillus atrophaeus DX-9 against potato CS offers an alternative biocontrol method that can improve both soil microbial community and properties.This study provides insight into the potential mechanisms by which microbial inoculants can control CS disease.

关 键 词：Bacillus atrophaeus Solanum tuberosum BIOCONTROL Common scab Soil properties Microbial community 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]