微量凝集试验与试管凝集试验检测布鲁氏菌病效果比较  

Comparison of efficiency of serum microagglutination test and serum tube agglutination test in detection of brucellosis

作　　者：关娜 塔娜[1,2] 姜海 左柯铭[1] 高静 蔺冰冰[1] 温永俊 Guan Na;Ta Na;Jiang Hai;Zuo Keming;Gao Jing;Lin Bingbing;Wen Yongjun(Inner Mongolia Agricultural University,Hohhot 010010,Inner Mongolia,China;Inner Mongolia Autonomous Region Center for Disease Control and Prevention(Inner Mongolia Academy of Prevention Medicine),Hohhot 010031,Inner Mongolia,China;National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,National Institute for Communicable Disease Controland Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Baotou Medical College,Inner Mongolia University of Science&Technology,Baotou 014040,Inner Mongolia,China)

机构地区：[1]内蒙古农业大学,内蒙古呼和浩特010010 [2]内蒙古自治区疾病预防控制中心(内蒙古自治区预防医学科学院),内蒙古呼和浩特010031 [3]传染病溯源预警与智能决策全国重点实验室,中国疾病预防控制中心传染病预防控制所,北京102206 [4]内蒙古科技大学包头医学院,内蒙古包头014040

出　　处：《疾病监测》2025年第3期371-375,共5页Disease Surveillance

基　　金：国家自然科学基金资助项目—国际(地区)合作与交流项目(No.82361148725)。

摘　　要：目的 评价微量凝集试验在布鲁氏菌病(布病)监测中的实用价值,为微量凝集试验能否在布病监测工作中应用提供参考依据。方法 从内蒙古自治区疾病预防控制中心门诊部收集疑似布病病例血清251份,健康血清28份。在相同实验条件下对标准血清及279份血清样品分别进行微量凝集试验与试管凝集试验并对实验结果进行对比分析。结果 标准血清和健康血清两种方法检测结果完全相同。疑似布病病例血清试管凝集与微量凝集试验结果效价完全符合率为80.48%。279份样品的微量凝集与试管凝集效价完全符合率为82.44%,对疑似布病病例血清试管凝集试验与微量凝集试验结果进行配对t检验,差异无统计学意义(t=0.122,P>0.05)。结论 微量凝集与试管凝集两种检测方法差异无统计学意义,但微量凝集法相对于试管凝集具有更多优点,因此,微量凝集试验更适宜在我国布病大量样本监测、诊断中推广应用。Objective Evaluate the practical value of the microagglutination test in brucellosis surveillance,and provide reference evidence for determining whether microagglutination test can be applied in brucellosis monitoring programs.Methods A total of 251 serum samples of suspected brucellosis patients and 28 serum samples of healthy people were collected from the outpatient department of Inner Mongolia Center for Disease Control and Prevention.Under the same experimental conditions,microagglutination test and tube agglutination test were performed by using standard serum samples and 279 serum samples respectively,and the results were compared and analyzed.Results The two tests shows same results in the detections of standard serum samples and healthy serum samples.For the serum samples from suspected brucellosis cases,the consistant rate of titers between tube agglutination test and microagglutination test was 80.48%.Among 279 samples,the titer consistant rate between microagglutination test and tube agglutination tests reached 82.44%.A paired T-test was conducted to compare the results of serum tube agglutination test and serum microagglutination test for suspected brucellosis cases and showed no significant difference between the two results(t=0.122,P>0.05).Conclusion There was no significant difference in efficiency between serum microagglutination test and serum tube agglutination test,but serum microagglutination test has more advantages compared with serum tube agglutination tests,so serum microagglutination test is more suitable for promoting the application of the test in the monitoring and diagnosis of a large number of brucellosis samples in China.

关 键 词：布鲁氏菌病 微量凝集试验 试管凝集试验 对比 血清学 

分 类 号：R211[医药卫生—中医学] R446.6