光肩星天牛刻槽和人工模拟刻槽对沙枣泌胶代谢物的影响  

The effects of Asian longhorned beetle(Anoplophora glabripennis)oviposition scar and artificially simulated oviposition scar on gum metabolism in Russian olive(Elaeagnus angustifolia)

作　　者：齐若涵 任利利[1] 郝柯宇 裴佳禾 骆有庆[1] QI Ruo-Han;REN Li-Li;HAO Ke-Yu;PEI Jia-He;LUO You-Qing(Beijing Key Laboratory for Forest Pest Control,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]北京林业大学林木有害生物防治北京市重点实验室,北京100083

出　　处：《应用昆虫学报》2025年第1期80-92,共13页Chinese Journal of Applied Entomology

基　　金：国家自然科学基金(32371886);国家重点研发计划(2022YFD1401000)。

摘　　要：【目的】沙枣Elaeagnus angustifolia可以吸引光肩星天牛Anoplophora glabripennis雌成虫前来刻槽产卵,并能够通过分泌树胶杀死刻槽内的卵或初孵幼虫,这说明沙枣有作为诱杀树来控制光肩星天牛种群的潜力。然而,光肩星天牛产卵刻槽行为对沙枣的泌胶及树胶化学成分的影响尚未明确。本研究旨在通过比较天牛刻槽和人工模拟刻槽条件下,沙枣分泌树胶的能力及树胶代谢物的变化,明确受到光肩星天牛危害后沙枣的代谢响应。【方法】收集光肩星天牛刻槽和人工模拟刻槽条件下15d的沙枣胶,称取重量并测定树胶中的可溶性蛋白、可溶性糖、还原性糖、黄酮、总酚和单宁的含量,进一步使用高效液相色谱串联质谱(LC-MS)对沙枣胶的小分子代谢物进行测定与分析。【结果】光肩星天牛刻槽后的沙枣的泌胶量显著高于模拟刻槽(P<0.05);在两种刻槽条件下,沙枣胶的可溶性蛋白、可溶性糖和还原性糖含量以及单宁、黄酮和总酚含量存在显著差异(P<0.01),其中天牛刻槽组测定的物质含量均显著高于人工模拟刻槽组(P<0.01)。在天牛刻槽与模拟刻槽比较组中,筛选得到80种差异代谢物,65种代谢物上调,15种代谢物下调,其中两种黄酮类化合物异鼠李素和水飞蓟宾极显著上调。差异代谢物的KEGG通路富集分析表明,光肩星天牛刻槽显著诱导了蛋白质消化和吸收、植物次生代谢产物的生物合成、氨酰-tRNA的生物合成、苯丙烷类物质的生物合成以及酪氨酸代谢通路。【结论】沙枣受到光肩星天牛产卵刻槽危害后,相较于人工模拟刻槽,分泌树胶的重量较高,树胶中次生代谢物含量升高,植物防御反应增强。本研究为进一步探究沙枣泌胶杀死天牛卵或幼虫的机制提供了初步的理论基础。[Aim] Adult female Asian longhorned beetles(Anoplophora glabripennis) create ovipositional scars and lay eggs on Russian olive(Elaeagnus angustifolia) trees,which respond by secreting enough gum to kill virtually all eggs and larvae.This indicates that the Russian olive has the potential to be used as a trap-tree to control the Asian longhorned beetle.However,the impact of the ovipositional behavior of the Asian longhorned beetle on the gum secretion of Russian olive trees has yet to be clarified.This study aims to compare the quantity of gum secreted,and its chemical composition,in response to natural,A.glabripennis oviposition scars,and artificial scars.[Methods] The gum of Russian olive trees was collected for 15 d from both natural oviposition scars and artificially simulated scars.The weight of gum from individual scars was measured,and the amount of soluble protein,soluble sugar,reducing sugar,flavonoids,total phenols,and tannins in gum from each scar type was determined.Furthermore,small molecular metabolites in the gum were analyzed using liquid chromatography-mass spectrometry(LC-MS).[Results] There was significant difference in both the weight,and composition,of gum produced in response to natural,and artificial,oviposition scars(P<0.05).The amounts of all measured substances in gum produced in response to actual oviposition scars was significantly higher than that produced in response to artificial scars(P<0.01).A total of 80 differential metabolites were identified in gum induced by both treatments,65 of which were up-regulated and 15 of which were down-regulated in natural scar gum relative to artificial scar gum.Two flavonoids,isorhamnetin and silibinin,were highly significantly up-regulated.KEGG pathway enrichment analysis of differential metabolites identified the top five pathways as,protein digestion and absorption,biosynthesis of plant secondary metabolites,aminoacyl-tRNA biosynthesis,biosynthesis of phenylpropanoids,and tyrosine metabolism,are of which are either directly,or indirectly,related

关 键 词：光肩星天牛 沙枣 刻槽 树胶 差异代谢物 

分 类 号：S763.42[农业科学—森林保护学]