亚致死浓度氯虫苯甲酰胺对斜纹夜蛾细胞免疫反应的影响  

The effect of sublethal concentrations of chlorantraniliprole on the cellular immunity of Spodoptera litura(Lepidoptera:Noctuidae)

作　　者：谢文琪 邓晓悦 张智慧 陶文彩 李庆[1] 蒋春先[1] XIE Wen-Qi;DENG Xiao-Yue;ZHANG Zhi-Hui;TAO Wen-Cai;LI Qing;JIANG Chun-Xian(College of Agronomy,Sichuan Agricultural University,Chengdu 611130,China)

机构地区：[1]四川农业大学农学院,成都611130

出　　处：《应用昆虫学报》2025年第1期179-191,共13页Chinese Journal of Applied Entomology

基　　金：国家现代农业产业技术体系四川创新团队(SCCXTD-2024-04)。

摘　　要：【目的】明确氯虫苯甲酰胺对斜纹夜蛾Spodoptera litura幼虫细胞免疫的亚致死效应,探究斜纹夜蛾抵御杀虫剂胁迫的机制。【方法】以斜纹夜蛾4龄幼虫为研究对象,分别用LC_(10)、LC_(20)和LC_(30)的氯虫苯甲酰胺处理,测定并分析幼虫血细胞形态、总数量以及吞噬、结节和包囊作用的变化。【结果】根据毒力测定结果,氯虫苯甲酰胺对斜纹夜蛾4龄幼虫处理48 h的LC_(10)、LC_(20)和LC_(30)分别为0.190、0.459和0.868μg/g。亚致死浓度氯虫苯甲酰胺在一定程度上促进幼虫血细胞总数量增加。斜纹夜蛾幼虫的血细胞类型包括原血细胞、浆血细胞、粒血细胞、珠血细胞以及类绛色细胞。在亚致死浓度氯虫苯甲酰胺处理下,斜纹夜蛾幼虫部分血细胞出现细胞皱缩、细胞膜变形、胞质空泡化、粒细胞脱粒及细胞核形变甚至凋亡等形态变化。LC_(10)氯虫苯甲酰胺在48 h后能抑制吞噬作用,LC_(20)氯虫苯甲酰胺在12 h后促进幼虫的吞噬作用,在6、48和72 h显著抑制(P<0.01)吞噬作用,LC_(30)氯虫苯甲酰胺能显著抑制斜纹夜蛾吞噬作用,但随着处理时间增加抑制作用逐渐减弱。LC_(10)、LC_(20)和LC_(30)氯虫苯甲酰胺均能显著(P<0.01)抑制斜纹夜蛾结节作用。LC_(10)氯虫苯甲酰胺能抑制斜纹夜蛾包囊作用,而LC_(20)和LC_(30)在12和24 h会促进包囊作用,在72 h显著(P<0.01)抑制。【结论】氯虫苯甲酰胺对斜纹夜蛾细胞免疫的亚致死效应表现为改变部分血细胞形态,一定程度上促进血细胞总数量增加,显著抑制结节作用。此外,随着药剂浓度和处理时间增加,亚致死浓度氯虫苯甲酰胺先促进后抑制吞噬和包囊作用。从昆虫免疫作用角度为未来害虫的控制和管理策略提供了理论依据。[Aim]To determine the sublethal effects of chlorantraniliprole on the cellular immunity of Spodoptera litura larvae and explore the mechanisms of involved in insecticide resistance.[Methods]Forth instar S.litura larvae were exposed to LC_(10),LC_(20) and LC_(30) concentrations of chlorantraniliprole.Hemocyte morphology,total hemocyte count,phagocytosis,nodulation,and encapsulation were measured to compare the effect of different sublethal exposure doses of chlorantraniliprole on the cellular immunity of S.litura larvae.[Results]Based on the bioassay,the LC_(10),LC_(20),and LC_(30)values of chlorantraniliprole for 4th instar S.litura larvae after 48 h of exposure were 0.190,0.459,and 0.868μg/g,respectively.A moderate increase in the total hemocyte count was observed following exposure to sublethal concentrations of chlorantraniliprole.S.litura larvae have several types of hemocytes including prohemocytes,plasmatocytes,granulocytes,spherulocytes,and oenocytoids.Exposure to sublethal concentrations of chlorantraniliprole resulted in various morphological changes to some hemocytes such as cell shrinkage,cell membrane deformation,vacuolation,granulocyte degranulation,cell nucleus deformation,cell nucleus apoptosis,among others.Exposure to LC_(10 )chlorantraniliprole for 48 h inhibited phagocytosis.Exposure to LC_(20) chlorantraniliprole for 12 h promoted phagocytosis,while exposures of 6,48,and 72 h significantly (P<0.01) inhibited phagocytosis.Exposure to LC_(30) chlorantraniliprole significantly inhibited phagocytosis,but the inhibitory effect gradually weakened with increasing treatment time.Exposure to LC_(10),LC_(20),and LC_(30 )chlorantraniliprole all significantly (P<0.01) inhibited nodulation in S.litura.LC_(10 )chlorantraniliprole exposure inhibited encapsulation,while LC_(20) and LC_(30) promoted encapsulation after 12 and 24 h exposure.However,longer exposure times significantlyinhibited encapsulation (P<0.01).[Conclusion]The results of this study indicate that sublethal doses of chlorantraniliprole affect

关 键 词：斜纹夜蛾 氯虫苯甲酰胺 亚致死效应 血细胞形态 血细胞总数量 吞噬作用 结节作用 包囊作用 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]