四种生物农药对美国白蛾的复配增效作用研究  

Synergistic effect of four biotic pesticides on Hyphantria cunea

作　　者：李强 陈文帮 蔡婷 王张远 陈敏 LI Qiang;CHEN Wen-Bang;CAI Ting;WANG Zhang-Yuan;CHEN Min(Key Laboratory Beijing Control Forest Pest,College of Forestry,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]北京林业大学林学院,林木有害生物防治北京市重点实验室,北京100083

出　　处：《应用昆虫学报》2025年第1期202-210,共9页Chinese Journal of Applied Entomology

基　　金：北京林业大学大学生创新创业训练计划(S202210022007)。

摘　　要：【目的】明确灭幼脲、虫酰肼、苦参碱和印楝素4种生物农药对美国白蛾Hyphantria cunea幼虫的杀虫活性和混配后的联合药效,为药剂的林间复合应用提供一定参考。【方法】采用人工饲料涂布法对美国白蛾4龄幼虫进行了4种单剂和混合药剂的室内毒力测定,采用共毒因子法筛选具有增效作用的最佳配伍,再用共毒系数法确定混配的最佳比例。【结果】4种生物农药对美国白蛾4龄幼虫的毒力大小顺序为印楝素>灭幼脲>苦参碱>虫酰肼,施药96 h后的致死中浓度(Median lethal concentration,LC_(50))依次为1.732、23.702、30.160和54.296 mg·L^(﹣1)。灭幼脲和虫酰肼以致死中浓度混配后表现出显著增效作用,且体积比为9∶1时增效作用最显著,其共毒系数为278.462,该复配组合处理美国白蛾幼虫的致死中时间(Median lethal time,LT_(50))分别比仅用虫酰肼和灭幼脲时缩短了0.704和1.281 d。其他混配组合均表现为拮抗或相加作用。【结论】4种生物农药对美国白蛾4龄幼虫均具有较高的杀虫活性,但仅有灭幼脲和虫酰肼按照一定比例混配具有显著增效作用。当灭幼脲和虫酰肼浓度分别为23.702和54.296 mg·L^(﹣1),且复配体积比为9∶1时杀虫效果最好。[Aim]To clarify the insecticidal activity,and combined efficacy,of four biotic pesticides,chlorbenzuron,tebufenozide,matrine,and azadirachtin,on Hyphantria cunea larvae.[Methods]The toxicity of four pesticides,both when applied separately and as a mixture,on the 4th instar larvae of H.cunea was determined using a surface contamination bioassay and an artificial diet.First,the co-toxicity factor method was used to identify the combination with the best synergistic effect,after which the co-toxicity coefficient method was used to identify the optimal mixing ratio.[Results]The relative toxicity of the four biotic pesticides was azadirachtin>chlorbenzuron>matrine>tebufenozide.The median lethal concentration (LC_(50)) 96 h after application was 1.732,23.702,30.160,and 54.296 mg·L~(-1),respectively.A mixture of chlorbenzuron and tebufenozide had significant synergistic effects at lethal concentrations,and this was most significant at a ratio of 9∶1,with a co-toxicity coefficient of 278.462.The median lethal time (LT_(50)) of this combination was 0.704 and 1.281 d less,respectively,than that for tebufenozide or chlorbenzuron.Other pesticide mixtures displayed antagonistic or additive effects.[Conclusion]The four biotic pesticides tested have high insecticidal activity against 4th instar H.cunea larvae,but only a mixture of chlorbenzuron and tebufenozide had significant synergistic effects.The best results were achieved at concentrations of chlorbenzuron and tebufenozide of 23.702 and 54.296 mg·L~(-1),respectively,and at a volume ratio of 9∶1.

关 键 词：美国白蛾 生物农药 共毒因子 共毒系数 增效作用 

分 类 号：S763.42[农业科学—森林保护学]