不同体色茶翅蝽成虫外部形态结构变化的几何形态学分析  

Geometric analysis of morphological variation between different body color biotypes of Halyomorpha halys

作　　者：潘鹏亮 张方梅 洪枫 郭世保 周洲 乔利 尹健 耿书宝 PAN Peng-Liang;ZHANG Fang-Mei;HONG Feng;GUO Shi-Bao;ZHOU Zhou;QIAO Li;YIN Jian;GENG Shu-Bao(School of Agronomy,Xinyang Agriculture and Forestry University,Xinyang 464000,China;Xinyang Academy of Ecological Research,Xinyang 464000,China)

机构地区：[1]信阳农林学院农学院,信阳464000 [2]信阳生态研究院,信阳464000

出　　处：《应用昆虫学报》2025年第1期211-227,共17页Chinese Journal of Applied Entomology

基　　金：河南省中央引导地方科技发展基金项目(Z20221341063);河南省高等学校重点科研项目(19A210021,24B210012);信阳生态研究院开放基金(2023XYQN08);信阳农林学院科技创新团队(KJCXTD202001,XNKJTD-007)。

摘　　要：【目的】自然界中有不同体色的茶翅蝽Halyomorpha halys,本文利用几何形态学方法对不同体色和不同性别茶翅蝽成虫外部形态差异进行分析,明确其体色分化是否存在形态学上的差异。【方法】利用平板扫描仪获取茶翅蝽前胸背板、前翅和后翅图像;通过tps Dig软件进行标记点和半标记点的获取;在Morpho J中进行普氏拟合(Procrustes fit)后,得到几何中心大小(Centroid size)和普氏坐标(Procrustes coordinates),并在SPSS中用于各部位大小变化的分析;对不同体色类型和性别等数据合并后,进行形状变化分析;最后通过普氏坐标与几何中心大小进行回归分析,其产生的残差用于评估移除异速生长的形状差异,并进行交叉判别分析。【结果】在几何中心大小方面,不同体色茶翅蝽雌性前胸背板(P_(标记点)=0.005,P_(半标记点)=0.003)和后翅(P_(标记点)=0.045)、雄性前胸背板(P_(标记点)=0.042)差异显著,相同体色不同性别茶翅蝽前胸背板(P_(浅色体型)<0.050,P_(深色体型)<0.001)、前翅(P_(深色体型)=0.020)和后翅(P_(两种体色类型)<0.050)差异显著。在平均形状变化上,深色体型雌雄间前胸背板差异显著(P<0.050),交叉判别正确率分别为90%和80%;雌性在不同体色上差异显著(P<0.050),利用前胸背板标记点和半标记点,以及前翅标记点判别正确率分别超过90%和76%。以体色和性别作为独立处理时,利用前胸背板进行交叉判别,雌性正确率大于90%,利用前翅标记点时深色雌性正确率最高达到100%,深色雄性最高为91.67%。去除异速生长后,标记点方法除了后翅外,其它部位判别正确率均有提高。【结论】利用几何形态学方法可以明确茶翅蝽体色变化与局部形态变化关系,可以用于种内不同性别间差异分析。[Aim]To use geometric morphometry to determine whether there are differences in morphology between the different color biotypes of Halyomorpha halys adults.[Methods]A flatbed scanner (Microtek MRS-9600TFU2L) was used to capture images of the pronotum,forewing,and hindwing of adult H.halys.Data on landmarks and semi-landmarks were obtained by digitizing these and using the draw curves function in tps Dig2.After Procrustes fit in Morpho J,centroid size and Procrustes coordinates were calculated and imported into SPSS for estimating size variation.After collating the data from different body-color types and genders,shape variation was analyzed using Morpho J software.Finally,regression was performed on the procrustes coordinates and centroid size.The residuals were used to assess differences in shape independent of size,and for a cross-validation test.[Results]Dark and light-colored biotypes differed significantly in the size of the pronotum (P_(landmark)=0.005,P_(semi-landmark)=0.003) and hindwings (P_(landmark)=0.045) of females and in the pronotum (P_(landmark)=0.042)of males.There was also significant sexual size dimorphism in the pronotum (P_(light-color)<0.050,P_(dark-color)<0.001),forewings(P_(dark-color)=0.020) and hindwings (P_(both color)<0.050) within the same biotype.There was sexual dimorphism in the shape of the pronotum (P<0.050)in the dark-colored biotype.Cross-validation indicated that 90%of males and 80%of females were correctly classified.Female morphology differed significantly between biotypes (P<0.050).Landmarks and semi-landmarks on the pronotum correctly classified>90%of specimens,whereas those from the forewings correctly classified>76%.When biotype and sex were analyzed as independent factors,cross-validation of pronotum data indicated that>90%of all females had been correctly classified.Data from forewing landmarks correctly classified 100%of females,and 91.67%of males from the dark-color biotype.Controlling for allometric effects further improved the results,except for hindwing data.[Conc

关 键 词：茶翅蝽 体色 形态变化 标记点 半标记点 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]