一种鳞翅目昆虫卵计数的方法及应用  

A method for counting lepidopteran insect eggs and its application

作　　者：訾化彬 冀睿婕 潘语涵 白俊青 李奎 蔚江涛 郑紫青 杨云秋[1] ZI Hua-Bin;JI Rui-Jie;PAN Yu-Han;BAI Jun-Qing;LI Kui;WEI Jiang-Tao;ZHENG Zi-Qing;YANG Yun-Qiu(State Key Laboratory of Tea Plant Biology and Utilization,Anhui Agricultural University,Hefei 230036,China;Xianning Academy of Agricultural Sciences,Xianning 437100,China;Yangling Hesheng Irradiation Technologies Co.,Ltd.,Xianyang 712100,China)

机构地区：[1]安徽农业大学,茶树生物学与资源利用国家重点实验室,合肥230036 [2]咸宁市农业科学院,咸宁437100 [3]杨凌核盛辐照技术有限公司,咸阳712100

出　　处：《应用昆虫学报》2025年第1期253-260,共8页Chinese Journal of Applied Entomology

基　　金：安徽省优秀科研创新团队项目(2022AH010055);国家重点研发计划课题(2021YFD1601105);国家自然科学基金(32371581)。

摘　　要：【目的】鳞翅目昆虫卵的数量是昆虫科学研究中一个非常重要的指标,但是部分鳞翅目昆虫产卵数量难以统计,亟需一种比较便捷的方法进行统计。【方法】通过对不同数量和重量的灰茶尺蠖Ectropis grisescens和斜纹夜蛾Spodoptera litura卵进行测量和分析,得出了卵数和卵重之间的关系并建立对应的线性模型,通过试验验证了卵数-卵重线性模型的精确性、有效性和适用性。【结果】建立的灰茶尺蠖W^(+)品系、灰茶尺蠖W^(-)品系以及斜纹夜蛾的卵数-卵重线性回归方程分别为Y=0.0776X-0.2640(R^(2)>0.99)、Y=0.0865X-0.0877(R^(2)>0.99)和Y=0.0537X-0.1845(R^(2)>0.99),使用3组线性拟合方程所预测的卵数相对误差均在3%以内;通过试验验证,在卵的1-4 d使用此方法最为精确,且使用此方法对卵的孵化率没有显著影响(P=0.364);根据不同品系的卵数-卵重线性拟合差异还可以进行品系区分。【结论】成功开发了一种鳞翅目昆虫卵计数的新方法,建立了卵数-卵重线性模型,为鳞翅目害虫的防治和管理提供了可靠的理论和实际应用基础。[Aim]Accurate quantification of lepidopteran insect eggs is crucial for insect research.However,counting eggs in certain Lepidopteran species can be challenging,necessitating a more convenient method.[Methods]This study measured and analyzed eggs of varying quantities and weights from Ectropis grisescens and Spodoptera litura.We established a precise linear model describing the relationship between egg numbers and their weights,validating its accuracy,effectiveness,and applicability through experiments.[Results]Linear regression equations for egg number-weight relationships in W~+and W~-strains of tea,geometrid,and tobacco cutworm were as follows:Y=0.077 6X-0.264 0 (R~2>0.99),Y=0.086 5X-0.087 7(R~2>0.99),and Y=0.053 7X-0.184 5 (R~2>0.99),respectively.The relative error in egg predictions using these equations remained under 3%.Experimental validation indicated the method had the highest accuracy for egg counts on days 1 to 4,without significantly affecting hatching rates (P=0.364).Moreover,differences in linear fittings between the different strains allowed for practical strain differentiation.[Conclusion]This study introduces an innovative method for counting lepidopteran insect eggs and establishes a reliable theoretical and practical foundation for managing and controlling lepidopteran pests.

关 键 词：鳞翅目 卵数 线性模型 品系区分 害虫防治 

分 类 号：S433[农业科学—农业昆虫与害虫防治]