生活饮用水中甲醛的柱后衍生-高效液相色谱-荧光测定法  

Determination of formaldehyde in drinking water by post column derivatization-high performance liquid chromatography-fluorimetry

作　　者：左家信[1] 陈东洋[1] ZUO Jia-xin;CHEN Dong-yang(Department of Physical and Chemical Analsis,Hunan Center for Disease Control and Prevention,Changsha,Hunan 410153,China)

机构地区：[1]湖南省疾病预防控制中心理化科,长沙410153

出　　处：《环境与健康杂志》2025年第3期247-249,共3页Journal of Environment and Health

基　　金：湖南省自然科学基金(2021JJ70085);湖南省卫生健康委科研项目(B20160073,C20190035,D202312069311)。

摘　　要：目的建立生活饮用水中甲醛的柱后衍生-高效液相色谱-荧光测定法。方法水样经微孔滤膜过滤,直接进样,采用XBridge^(TM)C_(18)(250 mm×4.6 mm,3.5μm)色谱柱进行分离,柱后衍生,荧光检测器检测,外标法定量。结果在0.05~10 mg/L的浓度范围内,所得甲醛的回归方程为y=5.6944x+0.0397,相关系数r=0.9999,具有良好的线性关系。该方法的检出限为0.01 mg/L,定量下限为0.05 mg/L;平均回收率为98.0%~104.0%,RSD为0.4%~1.1%。结论该方法具有操作简便、精密度好、回收率高等特点,适用于生活饮用水中甲醛的大批量检测。Objective To establish a method for the determination of formaldehyde in drinking water by post column derivatization-high performance liquid chromatography-fluorimetry.Methods The water samples were through the membrane and injected directly,separated by using XBridge^(TM)C_(18)(250 mm×4.6 mm,3.5μm)chromatography column to react generates new product,detected with fluorescence detector,quantified byexternal standard method.Results Formaldehyde had a good linear relationship with peak area in the range of 0.05-10 mg/L,the correlation coefficient r was 0.9999,the limit of detection(LOD)was 0.01 mg/L and the limit of quantification(LOQ)was0.05 mg/L.The recovery rates were 98.0%-104%with the relative standard deviations(RSD)were 0.4%-1.1%(n=6)at the spiked levels of 0.50,1.0 and 5.0 mg/L.Conclusion This method has the characteristics of simple operation,good precision and high recovery rate,and is applicable to the determination of large batches of samples,which is easy to promote and apply.

关 键 词：甲醛 饮用水 柱后衍生 高效液相色谱法 荧光法 

分 类 号：O657.6[理学—分析化学]