逆流训练对暗纹东方鲀免疫和抗氧化能力的影响  

Effects of upstream training on immunity and antioxidant capacity of Takifugu obscurus

作　　者：顾玲玲 李霞 冯广朋[1,2] 韩志强 GU Lingling;LI Xia;FENG Guangpeng;HAN Zhiqiang(East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai Engineering Research Center of Fisheries Resources Enhancement and Ecological Restoration of the Yangtze Estuary,Shanghai 200090,China;School of Fishery,Zhejiang Ocean University,Zhoushan 316022,Zhejiang,China)

机构地区：[1]中国水产科学研究院东海水产研究所,上海长江口渔业资源增殖和生态修复工程技术研究中心,上海200090 [2]浙江海洋大学水产学院,浙江舟山316022

出　　处：《生态学杂志》2025年第4期1276-1283,共8页Chinese Journal of Ecology

基　　金：国家重点研发计划“蓝色粮仓科技创新”重点专项(2020YFD0900805);上海长鱼长江水生生物保护基金会项目(2021CL03)资助。

摘　　要：在水生生物增殖放流前开展生境适应性的运动训练是提高苗种野外成活率的关键环节之一。为探究适宜的放流前运动强化技术,通过设置不同的训练强度(0、0.75和1.5 BL·s^(-1))和时间(7、14和28 d)的逆流训练试验,计算了暗纹东方鲀的成活率(SR)、肥满度(CF)、肝体比(HSI)和心脏指数(CI),测定了暗纹东方鲀(Takifugu obscurus)的血清溶菌酶(lysozyme,LZM)、皮质醇(cortisol,COR)、超氧化物歧化酶(superoxide dismutase,SOD)、乳酸脱氢酶(lactate dehydrogenase isozymes,LDH)和糖原(glycogen,Gn)水平的变化。结果表明:训练28 d后0.75 BL·s^(-1)训练组的SR高于对照组和1.5 BL·s^(-1)训练组;训练组LZM、COR、SOD、LDH与Gn均显著高于对照组(P<0.05),而训练组之间无显著差异(P>0.05);LZM、COR随着训练时间的延长先上升后下降,LDH随着训练时间的延长而升高;适宜的逆流训练(训练强度为0.75 BL·s^(-1),训练时间为14 d)可有效提高暗纹东方鲀的免疫酶活性,对提升放流成活率和放流成效有促进作用。Conducting exercise training of habitat adaptability before the enhancement and releasing of aquatic organisms is one of the key links to improve the survival rate of larval and juvenile fishes in the wild.To investigate the suitable exercise strengthening techniques before releasing,we examined survival rate(SR),condition factor(CF),hepatosomatic index(HSI)and cardiac index(CI)of Takifugu obscurus under different training intensities(0,0.75,1.5 BL·s^(-1))and training time(7,14,and 28 days)in the upstream training experiments.The changes of lysozyme(LZM),cortisol(COR),superoxide dismutase(SOD),lactate dehydrogenase lysozyme(LDH),and glycogen(Gn)levels in the serum of T.obscurus were measured.The results showed that the SR in the 0.75 BL·s^(-1) training group was higher than that of the control group and the 1.5 BL·s^(-1) training group after 28 days.The contents of LZM,COR,SOD,LDH,and Gn in all training groups were significantly higher than that in control group(P<0.05),without significant variations among the training groups(P>0.05).Moreover,the contents of LZM and COR first increased and then decreased with the extension of training time.The content of LDH also increased with the extension of training time.In summary,the appropriate upstream training(training intensity of 0.75 BL·s^(-1) with 14 days)can effectively improve the immune enzyme activity of T.obscurus,and enhance the survival rate and efficiency of releasing.

关 键 词：暗纹东方鲀 逆流训练 增殖放流 免疫性能 

分 类 号：S917.4[农业科学—水产科学]