脂多糖通过PI3K/AKT和Wnt/β-catenin信号通路对BESCs增殖和凋亡的影响  

The effects of lipopolysaccharide on proliferation and apoptosis of BESCs through the PI3K/AKT and Wnt/β-catenin signaling pathways

作　　者：董俊升 王梓 李汉卿[1,2,3] 郑芳玲 张敏 郭龙[1,2,3] 刘康军[1,2,3] 崔璐莹 王亨 李建基[1,2,3] DONG Junsheng;WANG Zi;LI Hanqing;ZHENG Fangling;ZHANG Min;GUO Long;LIU Kangjun;CUI Luying;WANG Heng;LI Jianji(Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses/College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China;Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education,Yangzhou 225009,China;International Research Laboratory of Prevention and Control of Important Animal infectious Diseases and Zoonotic Diseases of Jiangsu Higher Education Institutions,Yangzhou 225009,China)

机构地区：[1]扬州大学兽医学院江苏省动物重要疫病与人畜共患病防控协同创新中心,江苏扬州225009 [2]教育部农业与农产品安全国际合作联合实验室,江苏扬州225009 [3]江苏省高校动物重要疫病和重要人兽共患病防控技术国际合作联合实验室,江苏扬州225009

出　　处：《中国兽医学报》2025年第1期100-106,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金项目资助项目(32072937,32102735);江苏省自然科学基金资助项目(BK20210808);江苏现代农业产业技术体系建设专项基金资助项目(JATS[2023]456);江苏省333高层次人才培养工程资助项目,高等学校学科创新引智计划资助项目(D18007);江苏高校优势学科建设工程资助项目(PAPD)。

摘　　要：通过脂多糖(LPS)处理原代奶牛子宫内膜基质细胞(bovine endometrial stromal cells,BESCs),利用流式细胞术检测细胞凋亡率,利用CCK-法检测细胞活性,利用细胞划痕实验观察细胞迁移能力,利用荧光定量PCR法检测结缔组织生长因子(CTGF)、转化生长因子-β3(TGF-β3)和血管内皮生长因子(VEGF)mRNA的表达情况,利用Western blot技术检测PI3K/AKT和Wnt/β-catenin信号通路关键蛋白的表达情况,探讨了LPS诱导BESCs发生炎性反应时对其修复方面的影响。结果显示,LPS处理BESCs后,细胞活性明显降低(P<0.01);细胞迁移能力明显下降(P<0.05);BESCs凋亡率明显升高(P<0.01);CTGF和TGF-β3 mRNA表达量明显下降(P<0.01),但VEGF mRNA表达量上升(P<0.01);PI3K、AKT和GSK-3β蛋白磷酸化水平下降(P<0.05),c-Myc和Cyclin-D1蛋白表达量下降(P<0.01)。结果表明,LPS可抑制BESCs增殖,促进细胞凋亡,这可能与LPS抑制PI3K/AKT和Wnt/β-catenin信号通路的激活有关。In order to explore the effects of lipopolysaccharide(LPS)on the repair of bovine endometrial stromal cells(BESCs)during inflammatory response,BESCs were treated by LPS in this study.Cell apoptosis rate was detected using flow cytometry,cell viability was measured using the CCK-8 assay,cell migration ability was observed using a scratch assay,and the expression of connective tissue growth factor(CTGF),transforming growth factor-beta 3(TGF-β3)and vascular endothelial growth factor(VEGF)mRNA was measured using qRT-PCR.Additionally,the expression of key proteins in the PI3K/AKT and Wnt/β-catenin signaling pathways was assessed using Western blot analysis.The results showed that cell viability of BESCs significantly decreased(P<0.01),cell migration ability decreased(P<0.05),apoptosis rate of BESCs increased(P<0.01),CTGF and TGF-β3 mRNA expression levels decreased(P<0.01),while VEGF mRNA expression increased after treatment with LPS(P<0.01).The phosphorylation levels of PI3K,AKT and GSK-3βproteins decreased(P<0.05),as well as the expression levels of c-Myc and Cyclin-D1proteins also decreased(P<0.01).These results indicated that LPS can inhibit the proliferation of BESCs and promote cell apoptosis possibly through the inhibition of the PI3K/AKT and Wnt/β-catenin signaling pathways.

关 键 词：奶牛子宫内膜基质细胞 LPS PI3K/AKT WNT/Β-CATENIN 凋亡 增殖 

分 类 号：S852.4[农业科学—基础兽医学]