UPLC-MS/MS测定茅苍术中苍术内酯含量的研究  

Study of atractylolides determination in Atractylodes lancea(Thunb.)DC.by UPLC-MS/MS

作　　者：张文文 吴琴燕[2] 赵来成[2] 梁红芳 王建华[2] 陈露[2] ZHANG Wenwen;WU Qinyan;ZHAO Laicheng;LIANG Hongfang;WANG Jianhua;CHEN Lu(Zhenjiang Agricultural and Rural Bureau,Zhenjiang 212009,Jiangsu;Zhenjiang Institute of Agricultural Sciences in Hill Area of Jiangsu Province,Jurong 212405,Jiangsu)

机构地区：[1]镇江市农业农村局,江苏镇江212009 [2]江苏丘陵地区镇江农业科学研究所,江苏句容212405

出　　处：《浙江农业科学》2025年第4期923-927,共5页Journal of Zhejiang Agricultural Sciences

基　　金：镇江市“1+1+N”新型农业技术推广项目(ZJNJ〔2022〕04)。

摘　　要：建立茅苍术中苍术内酯Ⅰ、苍术内酯Ⅱ和苍术内酯Ⅲ超高效液相色谱-串联质谱(UPLC-MS/MS)检测方法。通过质谱特征扫描,各化合物均获得较高离子化效应离子对,样品在室温条件下,采用纯甲醇提取,液固比为20 mL·g^(-1),超声提取45 min,3种苍术内酯的提取量均达到较高水平,分别为621.33、201.32和275.97μg·g^(-1);利用加标回收试验,3种苍术内酯加标回收率为85.7%~106.2%,相对标准偏差为6.0%~9.7%,方法的检测限和定量限分别为18.2~32.2、61.0~111.2μg·L^(-1)。该方法可用于同时分析茅苍术中3种苍术内酯含量。A method for determination of atractylolide Ⅰ,atractylolide Ⅱ and atractylolide Ⅲ in Atractylodes lancea(Thunb.)DC.by ultra-high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)was developed and validated.Ion pairs with high ionization effect were obtained via feature scan of mass spectrometry.The samples were extracted with methanol at room temperature,the liquid-solid ratio was 20 mL·g^(-1),and the ultrasonic extraction time was 45 min.The extraction amount of the three atractylolides reached a high level,which were 621.33、201.32 and 275.97μg·g^(-1) respectively;The recoveries of the three atractylodes were in the range of 85.7%-106.2% with the relative standard deviations of 6.0%-9.7%.The detection limit and quantification limit of the method are 18.2-32.2μg·L^(-1) and 61.0-111.2μg·L^(-1),respectively.This method can be suitable for the simultaneous analysis of three atractylodes in Atractylodes lancea.

关 键 词：超高效液相色谱-串联质谱 茅苍术 苍术内酯 

分 类 号：S567.23[农业科学—中草药栽培]