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作 者:王红 洪方磊 郑博升 余宏傲[1,2] WANG Hong;HONG Fanglei;ZHENG Bosheng;YU Hongao(Wenzhou Vocational College of Science and Technology(Wenzhou Academy of Agricultural Sciences),Wenzhou 325006,Zhejiang;Wenzhou Key Laboratory of Horticultural Plant Breeding,Wenzhou 325006,Zhejiang)
机构地区:[1]温州科技职业学院(温州市农业科学研究院),浙江温州325006 [2]温州市园艺植物分子育种重点实验室,浙江温州325006
出 处:《浙江农业科学》2025年第4期945-951,共7页Journal of Zhejiang Agricultural Sciences
基 金:温州市农业高新园区开放性项目(KN20210006);温州市环大罗山省级现代农业园区科技支撑项目(WZDLS2021-01);温州市农业新品种选育协作组项目(ZX2024002-5)。
摘 要:试验主要开展瓯柑微芽嫁接的无菌砧木和接穗的获取,以及茎尖大小、嫁接方法对瓯柑试管内微芽嫁接成活的影响,并通过RT-PCR技术检测嫁接苗的黄龙病脱毒效果。结果表明,本地朱栾种子和广西枳壳当年萌芽率最高,0.5%TTC染色30 min可快速检测生活力。以广西枳壳种子为砧木,50%必洁仕消毒液消毒种子20 min,水+琼脂的培养基是最佳萌芽培养条件,广西枳壳当年无菌萌芽率达到43.5%。不同砧木品种微芽嫁接成活差异较大,试验中4个砧木品种微芽嫁接成活难易依次为朱栾>枳壳>香橙>红橘,接穗大小和嫁接方法均对微芽嫁接成活率有显著影响,采用0.2~0.4 mm的茎尖直径进行三角形芽接,接后30 d嫁接成活率42.5%,黄龙病脱除率41.6%。The experiment primarily focused on obtaining sterile rootstocks and scions for citrus micro-grafting,and investigated the effects of shoot tip size and grafting methods on the survival rate of in vitro micro-grafting in Ougan citrus.Additionally,the effectiveness of Huanglongbing(HLB)pathogen elimination in grafted seedlings was detected using RT-PCR technology.The results showed that local Zhuluan seeds and Guangxi trifoliate orange seeds exhibited the highest germination rate in the current year.Using Guangxi trifoliate orange seeds as rootstocks,the optimal germination conditions were achieved by disinfecting the seeds with 50% Bijieshi disinfectant for 10-20 minutes and culturing them in a water+agar medium,yielding a sterile germination rate of 43.5%.The survival rates of micro-grafting varied significantly among different rootstock varieties.Among the four rootstock varieties tested,the ease of micro-grafting survival followed the order:Zhuluan>trifoliate orange>fragrant orange>red tangerine.Triangular bud grafting was performed using a stem tip diameter of 0.2-0.4 mm.The survival rate of the stem tip was 42.5% after 30 days grafting.The removal rate of Huanglongbing was 41.6%.
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