稻纵卷叶螟不同发育阶段miRNA表达谱分析  

Analysis of miRNA expression profiles at different developmental stages of Cnaphalocrocis medinalis

作　　者：朱宇 刘洋 ZHU Yu;LIU Yang(Key Laboratory of Crop Breeding in South Zhejiang,Wenzhou Vocational College of Science and Technology,Wenzhou 325006,Zhejiang)

机构地区：[1]温州科技职业学院浙南作物育种重点实验室,浙江温州325006

出　　处：《浙江农业科学》2025年第4期979-985,共7页Journal of Zhejiang Agricultural Sciences

摘　　要：该文通过高通量测序技术分析了稻纵卷叶螟五龄幼虫和蛹中miRNA的表达情况,并对显著差异表达的miRNAs进行了靶基因的预测和功能富集分析。在稻纵卷叶螟的五龄幼虫和蛹的miRNA文库中,存在44个miRNAs。五龄幼虫和蛹文库之间差异表达显著的miRNAs有11个,对应的靶基因有756个。对靶基因进行功能分析,发现这些靶基因显著富集到14个GO条目。该研究不仅提供了稻纵卷叶螟五龄幼虫和蛹发育过程中miRNA表达谱和差异表达信息,也为miRNA调控昆虫发育的功能研究奠定了基础。This study analyzed the expression profiles of miRNAs in fifth-instar larvae and pupae of Cnaphalocrocis medinalis using high-throughput sequencing technology,and predicted the target genes of significantly differentially expressed miRNAs and performed functional enrichment analysis.In the miRNA library of fifth-instar larvae and pupae of C.medinalis,44 miRNAs were present.There were 11 differentially expressed miRNAs between the fifth-instar larval and pupal libraries,with a total of 756 predicted target genes.Functional analysis of the target genes showed that these target genes were significantly enriched to 14 GO terms.This study not only provided miRNA expression profile and differential expression information during the development of fifth-instar larvae and pupae of C.medinalis,but also laid a foundation for the study of the function of miRNA in regulating insect development.

关 键 词：稻纵卷叶螟 MIRNA 靶基因 

分 类 号：S435.112.1[农业科学—农业昆虫与害虫防治]