免疫分析方法在氧氟沙星残留检测中的研究进展  

Research progress of immunoassay methods in the detection of ofloxacin residues

作　　者：杨兴东 曲扬 胡骁飞[2] 李俐俐[1] YANG Xingdong;QU Yang;HU Xiaofei;LI Lili(Institute of Food and Drug Inspection,College of Life Science and Agriculture,Zhoukou Normal University,Zhoukou 466001,Henan;Key Lab for Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan)

机构地区：[1]周口师范学院生命科学与农学院食品药品检验检测中心,河南周口466001 [2]河南省农业科学院动物免疫学重点实验室,河南郑州450002

出　　处：《浙江农业科学》2025年第3期742-749,共8页Journal of Zhejiang Agricultural Sciences

基　　金：河南省科技攻关项目(212102110094)。

摘　　要：氧氟沙星(ofloxacin,OFLX)是一种主要的氟喹诺酮类(FQs)药物,因其抗菌性好、价格低廉而常被用于人类和动物的医疗保健中,但其残留物对人类健康造成的风险巨大,引起人们的高度重视。鉴于OFLX在食品和环境中有较多残留,建立对OFLX的即时检测方法势在必行。与传统的色谱、光谱检测技术相比,免疫分析法在特异性、检测效率、现场适用性和前处理的简便性等方面具有突出优势。该文综述了OFLX抗体的制备和免疫分析技术在OFLX残留检测中的应用现状,比较了酶联免疫法、免疫层析法、荧光免疫法、免疫传感器法、生物条形码免疫分析法、核酸适配体技术等方法的优缺点,并对OFLX免疫检测方法的发展趋势进行了展望,以期为该抗生素快速检测方法的建立和应用提供参考。Ofloxacin(OFLX),a major kind of fluoroquinolones(FQs),is commonly used in human and animal healthcare due to its antimicrobial properties and low cost.However,the risk to human health posed by its residues has attracted considerable attention.In view of the residues of OFLX in the food and environment,it is imperative to establish its immediate detection.Compared with traditional chromatographic and spectroscopic detection techniques,immunoassay has outstanding advantages in terms of specificity,detection efficiency,field applicability and simplicity of pre-treatment.This paper reviewed the preparation of OFLX antibodies and the current application of immunoassay techniques in OFLX residue detection,compared the advantages and disadvantages of enzyme-linked immunoassay,immunochromatographic method,fluorescence immunoassay,immunosensor,bio-barcode immunoassay,and nucleic acid aptamer,etc.,and looked forward to the development trend of the immunoassay methods of OFLX with the aim of providing a reference for the establishment and application of rapid antibiotic detection methods.

关 键 词：氧氟沙星 抗体 免疫分析 生物传感器 核酸适配体 

分 类 号：S859.796[农业科学—临床兽医学]