厚朴酚对急性坏死性胰腺炎大鼠小肠Cajal细胞自噬和小肠运动功能的影响  

Effects of magnolol on autophagy of interstitial Cajal cells and intestinal motility in acute necrotizing pancreatitis rats

作　　者：陈阳琴 姜昊文 齐文杰[1] 苗彬[1] Chen Yangqin;Jiang Haowen;Qi Wenjie;Miao Bin(Department of Infectious Disease,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China)

机构地区：[1]首都医科大学附属北京友谊医院感染内科,北京100050

出　　处：《中华胰腺病杂志》2025年第2期119-125,共7页Chinese Journal of Pancreatology

基　　金：国家自然科学基金(82274259、81774045);北京市自然科学基金(7152044)。

摘　　要：目的探讨厚朴酚对急性坏死性胰腺炎(ANP)大鼠小肠Cajal细胞自噬和小肠运动功能的影响。方法45只Wistar大鼠按数字表法随机分为对照组、ANP组和厚朴酚干预组,每组15只。采用雨蛙素腹腔注射的方法制备大鼠ANP模型,厚朴酚干预组在造模后30 min尾静脉注射20μg/kg厚朴酚乙醇溶液。造模12 h后取回肠组织行病理学检查并行病理学评分;采用碳末推进法测定小肠传输速率,制备离体小肠肌条测定肌条自主收缩波幅和频率;分别采用黄嘌呤氧化酶法、硫代巴比妥酸法、酶还原法试剂盒测定小肠组织氧化应激指标超氧化物歧化酶(SOD)活力、丙二醛(MDA)和一氧化氮(NO)含量;分离小肠平滑肌组织Cajal细胞,采用蛋白质免疫印迹法检测Cajal细胞自噬相关蛋白Beclin1、LC3Ⅱ、LC3Ⅰ、p62及p-Kit的表达;采用双重免疫荧光染色示踪Cajal细胞自噬形成。结果对照组、ANP组、厚朴酚干预组回肠组织病理评分分别为(0.33±0.52)、(4.83±0.41)、(3.50±0.55)分,ANP组较对照组显著升高,厚朴酚干预组较ANP组降低,差异有统计学意义(P值均<0.05)。ANP组大鼠小肠传输速率、自发性节律性收缩的波幅和频率较对照组显著减慢,而厚朴酚干预组小肠传输速率、自发性节律性收缩的波幅和频率较ANP组显著增加,差异均有统计学意义(P值均<0.05)。对照组、ANP组、厚朴酚干预组的SOD活力分别为(73.8±8.1)、(42.8±7.2)、(71.2±10.4)N/mg prot,NO含量分别为(1.72±0.26)、(3.19±0.43)、(1.94±0.23)μmol/g prot,MDA含量分别为(1.15±0.38)、(3.84±0.30)、(1.68±0.33)nmol/mg prot,ANP组较对照组SOD活力明显降低,NO含量和MDA含量显著增高,厚朴酚干预组较ANP组的SOD活力显著升高,NO含量以及MDA含量显著降低,差异均有统计学意义(P值均<0.01)。ANP组小肠Cajal细胞Beclin1蛋白水平、LC3Ⅱ/Ⅰ比值及p-Kit水平较对照组显著升高,p62蛋白表达水平明显降低,厚朴酚干预组Beclin1水�ObjectiveTo explore the effects of magnolol on autophagy in intestinal Cajal cells and intestinal motility in rats with acute necrotizing pancreatitis(ANP).MethodsForty-five Wistar rats were randomly divided into three groups by a random number table:control group,ANP group and magnolol intervention group,with 15 rats in each group.The ANP model was established by intraperitoneal injection of cerulein.The magnolol intervention group received a tail vein injection of 20µg/kg magnolol ethanol solution 30 minutes after modeling.After 12 hours,ileal tissues were collected for pathological examination and scoring.Intestinal transit rate was measured using the carbon powder propulsion method,and isolated intestinal muscle strips were prepared to assess amplitude and frequency of spontaneous contraction.Oxidative stress markers in intestinal tissues,including superoxide dismutase(SOD)activity,malondialdehyde(MDA)and nitric oxide(NO)levels,were measured using xanthine oxidase,thiobarbituric acid,and enzymatic reduction assay kits,respectively.Cajal cells were isolated from intestinal smooth muscle tissues,and the expression of autophagy-related proteins(Beclin1,LC3Ⅱ,LC3Ⅰ,p62)and p-Kit was detected by Western blot.Double immunofluorescence staining was used to trace autophagy in Cajal cells.ResultsThe pathological scores of ileal tissues in the control,ANP,and magnolol intervention groups were(0.33±0.52),(4.83±0.41),and(3.50±0.55),respectively.The score in ANP group was significantly higher than that in the control group,while the score in the magnolol intervention group was lower than that in the ANP group,with statistically significant differences(all P value<0.05).Intestinal transit rate,amplitude and frequency of spontaneous contraction in the ANP group were significantly slower than those in the control group,while these parameters in the magnolol intervention group were significantly improved compared to the ANP group,with statistically significant differences(all P value<0.05).SOD activity in the control,AN

关 键 词：胰腺炎 急性坏死性 自噬 小肠 厚朴酚 大鼠 

分 类 号：R285.5[医药卫生—中药学]