ATP对离体脊髓运动神经元突触传递的影响  

Effects of ATP on synaptic transmissions in spinal cord motoneurons in vitro

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作  者:陈在延 董萱 汪萌芽[1] CHEN Zaiyan;Dong Xuan;WANG Mengya(Cell Electrophysiology Laboratory,Wannan Medical College,Wuhu 241002,Anhui,China)

机构地区:[1]皖南医学院细胞电生理研究室,安徽芜湖241002

出  处:《皖南医学院学报》2025年第2期103-106,共4页Journal of Wannan Medical College

基  金:国家自然科学基金项目(31271155)。

摘  要:目的:观察三磷酸腺苷(ATP)对新生大鼠离体脊髓运动神经元(MN)下行激活和脊髓内在通路突触传递的影响。方法:选用SD大鼠乳鼠(9~12日龄)制备脊髓横切片,应用离体脊髓MN细胞内记录技术,记录电刺激(单脉冲,波宽0.1~0.3 ms,10次/分钟,15~100 V)同侧腹外侧索(iVLF)和同侧中央管周围区(iPCC)诱发MN产生兴奋性突触后电位(EPSP),即iVLF-EPSP和iPCC-EPSP,观察ATP灌流对iVLF-EPSP和iPCC-EPSP的影响。结果:①在6个稳定记录的MN,诱导出iVLF-EPSP并灌流10μmol/L ATP 15 min的MN有4个,在4个MN上观察到iVLF-EPSP的幅度被抑制(P<0.05)。②在6个稳定记录的MN,诱导出iPCC-EPSP并灌流10μmol/L ATP 15 min的MN有3个,在3个MN上观察到iPCC-EPSP的幅度被抑制(P<0.05),时程被缩短(P<0.05)。③在2个稳定记录的MN中,诱导出iPCC-EPSP并灌流10、100μmol/L ATP各15 min,观察到iPCC-EPSP幅度的平均抑制率分别为18%、54%。结论:ATP对脊髓运动神经元的iVLF-EPSP和iPCC-EPSP有浓度依赖性抑制作用。Objective:To explore the effects of ATP on synaptic transmissions,mediated by the spinal intrinsic regulation pathway and descending activation pathway to motoneuron(MN)in spinal cord slices.Methods:Neonatal SD rats(9-12-day-old)were used to prepare the spinal cord slices,and the ipsilateral ventrolateral funiculus(iVLF)and ipsilateral pericentral canal(iPCC)were electrically stimulated(single pulse,duration 0.1-0.3 ms,10 times/min,voltage 15-100 V)to induce excitatory postsynaptic potential(EPSP,iVLF-EPSP and iPCC-EPSP)in MN.ATP at required concentration was perfused and effects on spinal MN iVLF-EPSP and iPCC-EPSP were observed.Results:①In 6 steadily recorded MN,iVLF-EPSP was evoked and 10μmol/L ATP was perfused for 15 min in 4 MN.Inhibitory effect of ATP on iVLF-EPSP amplitude was observed in 4 MN(P<0.05);②In 6 steadily recorded MN,iPCC-EPSP was evoked and 10μmol/L ATP was perfused for 15 min in 3 MN.Inhibitory effects on amplitude(P<0.05)and duration(P<0.05)of iPCC-EPSP were observed in 3 MN;③In 2 steadily recorded MN,the amplitude of iPCC-EPSP was decreased after perfusion with 10 and 100μmol/L ATP respectively for 15 min,with average inhibition rate of 18%and 54%,respectively.Conclusion:ATP can inhibit iVLF-EPSP and iPCC-EPSP in the spinal cord motoneurons in vitro in concentration dependent fashion.

关 键 词:三磷酸腺苷 运动神经元 突触传递 脊髓 

分 类 号:R338.1[医药卫生—人体生理学] R-332[医药卫生—基础医学]

 

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