金丝桃苷电化学传感器创新实验教学设计  

Teaching Design of Innovative Experimental for Hyperoside Based on Electrochemical Sensor

作　　者：李小蓉[1] 龙旭[1] 闫浩 李佳佳 任艳茹 LI Xiaorong;LONG Xu;YAN Hao;LI Jiajia;REN Yanru(College of Pharmacy,Shaanxi University of Chinese Medicine,Xianyang 712046,Shaanxi,China)

机构地区：[1]陕西中医药大学药学院,陕西咸阳712046

出　　处：《实验室研究与探索》2025年第4期167-172,178,共7页Research and Exploration In Laboratory

基　　金：陕西中医药大学校级科研项目(2020GP08);陕西本科和高等继续教育教学改革研究(23BY119);陕西省教师教育改革与教师发展研究项目(SJS2023YB055)。

摘　　要：设计了基于纳米镍-多壁碳纳米管复合材料的电化学传感器测定金丝桃苷含量的创新实验。采用扫描电镜观察了修饰电极的表面形貌,用电化学阻抗谱研究了修饰电极的电化学性能,对实验条件进行了优化并用差分脉冲伏安法对金丝桃苷的含量进行了检测。在最佳条件下,传感器对金丝桃苷响应的线性范围为30~100.0nmol·L^(-1),检出限达到1.6nmol·L^(-1)。该传感器导电性好、灵敏度高、制作简单,将其用于黄蜀葵花中金丝桃苷含量的测定,回收率为98.3%~103.9%,具有一定的应用潜能。该实验集纳米材料和电化学传感器制备、中药有效成分的含量测定于一体,充分体现了创新型实验教学在激发学生的科研热情,培养学生创新思维、探究精神和科研素养中所起的重要作用。Relying on the teacher’s scientific research project,an experiment is innovatively designed to determine the content of hyperoside by electrochemical sensor based on nanonickel and multiwalled carbon nanotube composites modified electrode.The surface morphology of the modified electrode is studied by scanning electron microscopy,and the electrochemical performance of the modified electrode is investigated using electrochemical impedance spectroscopy.The experimental conditions are optimized and the content of hyperoside is detected by differential pulse voltammetry.Under the optimal conditions,the linear range of the sensor response to hyperoside is 3.0~100.0 nmol·L^(-1),and the detection limit is 1.6 nmol·L^(-1).The sensor has many advantages,such as good electrical conductivity,high sensitivity,and simple fabrication.It is used for the determination of hyperoside in Abelmoschus manihot samples with the recovery rate of 98.3%~103.9%,which has certain application potential.The experiment integrates nanomaterials,electrochemical sensors,and the determination of active ingredients in traditional Chinese medicine,which reflects the important role of experimental teaching in stimulating students’enthusiasm for scientific research and cultivating students’innovative thinking,inquiry spirit and scientific research literacy.

关 键 词：电化学传感器 纳米镍 多壁碳纳米管 金丝桃苷 创新实验 

分 类 号：G642.423[文化科学—高等教育学]