L-丙氨酰-L-谷氨酰胺二肽对杂交施氏鲟幼鱼生长和能量收支的影响  

Effects of L-Alanyl-L-Glutamine Dipeptide on Growth and Energy Balance of Juvenile Acipenser schrenckii♀×Acipenser baerii

作　　者：王晗雅 刘烊廷 杜飞 王帅[3] 陆绍霞 韩世成[2] 王常安[2] 刘红柏[2] WANG Hanya;LIU Yangting;DU Fei;WANG Shuai;LU Shaoxia;HAN Shicheng;WANG Chang’an;LIU Hongbai(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;Key Laboratory of Aquatic Animal Diseases and Immunity of Heilongjiang Province,Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China;Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国水产科学研究院黑龙江水产研究所,黑龙江省水产动物病害与免疫重点实验室,哈尔滨150070 [3]东北农业大学,哈尔滨150030

出　　处：《动物营养学报》2025年第4期2587-2598,共12页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家重点研发计划项目(2023YFD2400400-5);中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金资助(2023TD60,2023TD96);黑龙江省自然科学基金(LH2023C057);中国博士后基金(2022MD713817)。

摘　　要：本试验旨在探究饲料中添加L-丙氨酰-L-谷氨酰胺二肽(Ala-Gln)对杂交施氏鲟(Acipenser schrenckii♀×Acipenser baerii)幼鱼生长和能量收支的影响。选取450尾初始体重为(21.23±0.17)g的杂交施氏鲟,随机分成5组,每组3个重复,每个重复30尾。对照组(G1组)饲喂基础饲料,试验组分别饲喂在基础饲料中添加0.25%(G2组)、0.50%(G3组)、0.75%(G4组)和1.00%Ala-Gln(G5组)的饲料。试验期56 d。结果表明:1)随着饲料中Ala-Gln添加量的提高,试验鱼终末体重、增重率和肥满度逐渐提高,饲料系数逐渐降低,其中与G1组相比,G5组终末体重显著提高(P<0.05),G4组和G5组肥满度显著提高(P<0.05)。2)随着饲料中Ala-Gln添加量的提高,全鱼粗蛋白质含量呈现先降低后升高的变化趋势,其中G4组全鱼粗蛋白质含量最高;G5组全鱼粗脂肪含量最低,显著低于G2组(P<0.05)。3)饲料中添加Ala-Gln未改变全鱼氨基酸组成模式。G5组全鱼总氨基酸含量最高,显著高于G4组(P<0.05)。4)随着饲料中Ala-Gln添加量的提高,血清谷氨酰胺和丙氨酸含量均逐渐升高,与G1组相比,G3组、G4组和G5组血清谷氨酰胺和丙氨酸含量显著提高(P<0.05)。5)随着饲料中Ala-Gln添加量的提高,粪能和排泄能逐渐降低,其中与G1组相比,G3组、G4组和G5组粪能和排泄能显著降低(P<0.05);G5组生长能最高,显著高于G1组和G2组(P<0.05);同时,G5组代谢能显著低于G1组(P<0.05)。综上所述,饲料中添加适量Ala-Gln可提高杂交施氏鲟幼鱼终末体重和肥满度,促进机体蛋白质合成,并提高能量利用效率。在本试验条件下,杂交施氏鲟幼鱼饲料中以添加0.75%~1.00%Ala-Gln效果较佳。This experiment was conducted to investigate the effects of dietary supplementation of L-alanyl-L-glutamine dipeptide(Ala-Gln)on growth and energy balance of juvenile Acipenser schrenckii♀×Acipenser baerii.A total of 450 hybrid sturgeons with an initial body weight of(21.23±0.17)g were randomly divided into 5 groups with 3 replicates per group and 30 fish per replicate.Fish in the control group(G1 group)were fed a basal diet,and those in the experimental groups were fed the basal diet supplemented with 0.25%(G2 group),0.50%(G3 group),0.75%(G4 group)and 1.00%Ala-Gln(G5 group),respectively.The experiment lasted for 56 days.The results showed as follows:1)with the increase of dietary Ala-Gln supplemental level,the final body weight,weight gain rate and condition factor of fish were gradually increased,and the feed coefficient was gradually decreased.Compared with G1 group,the final body weight in G5 group was significantly increased(P<0.05),and the condition factor in G4 and G5 groups was significantly increased(P<0.05).2)With the increase of dietary Ala-Gln supplemental level,the crude protein content in whole fish showed a trend of decreasing first and then increasing,and the crude protein content in whole fish in G4 group was the highest;the ether extract content in whole fish in G5 group was the lowest,and significantly lower than that in G2 group(P<0.05).3)Dietary supplementation of Ala-Gln did not change the amino acid composition pattern of whole fish.The total amino acid content in whole fish in G5 group was the highest,and significantly higher than that in G4 group(P<0.05).4)With the increase of dietary Ala-Gln supplemental level,the serum glutamine and alanine contents were gradually increased,and compared with G1 group,the serum glutamine and alanine contents in G3,G4 and G5 groups were significantly increased(P<0.05).5)With the increase of dietary Ala-Gln supplemental level,the fecal energy and excretion energy were gradually decreased,and compared with G1 group,the fecal energy and excretion energy

关 键 词：L-丙氨酰-L-谷氨酰胺二肽 施氏鲟 生长性能 能量收支 

分 类 号：S963[农业科学—水产养殖]