犬源罗伊氏乳杆菌LRA7对比格犬短链脂肪酸含量、肠道菌群及代谢组的影响  

Effects of Canine-Derived Lactobacillus reuteri LRA7 on Short-Chain Fatty Acid Contents,Intestinal Flora and Metabolome in Beagle Dogs

作　　者：张媛媛 赵梦迪 李悦垚 李光玉 ZHANG Yuanyuan;ZHAO Mengdi;LI Yueyao;LI Guangyu(College of Animal Science and Technology,Qingdao Agricultural University,Qingdao 266109,China;College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]青岛农业大学动物科技学院,青岛266109 [2]吉林农业大学动物科技学院,长春130118

出　　处：《动物营养学报》2025年第4期2648-2660,共13页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：青岛农业大学高层次人才科研启动基金(1121021)。

摘　　要：本试验旨在探讨犬源罗伊氏乳杆菌LRA7对犬短链脂肪酸含量、肠道菌群和代谢组的影响。选取年龄为(2.50±0.56)岁成年健康比格犬18只,随机分为3组,每组6个重复,每个重复1只。3组分别为对照组(CK组)和2个益生菌添加组(LR组、HR组),其中CK组饲喂基础饲粮,LR组和HR组在饲喂基础饲粮的同时,分别补充1×10^(8)和1×10^(10) CFU/mL的犬源罗伊氏乳杆菌LRA7,每只犬每天口服给药2 mL。预试期7 d,正试期28 d。结果显示:1)与CK组相比,LR组和HR组犬粪便中乙酸和丙酸含量显著增加(P<0.05)。2)各组之间肠道菌群α多样性和β多样性无显著差异(P>0.05)。3)由属水平物种丰度图分析表明,与CK组相比,益生菌添加组乳杆菌属相对丰度占比增加,但无显著差异(P>0.05);肠道菌群t检验和LEfSe差异分析结果显示,LR组拟杆菌门、普雷沃氏菌属、韦荣氏菌科相对丰度增加,梭菌目相对丰度显著降低(P<0.05)。4)与CK组相比,LR组差异代谢物主要注释到氨基酸代谢通路,HR组差异代谢物主要注释到脂质代谢通路。综上所述,犬源罗伊氏乳杆菌LRA7可增加比格犬粪便中乙酸和丙酸含量,改善肠道菌群组成,显著上调氨基酸代谢通路和脂质代谢通路,从而改善比格犬的肠道健康。The aim of this study was to investigate the effects of canine-derived Lactobacillus reuteri LRA7 on short-chain fatty acid contents,intestinal flora and metabolome in dogs.A total of 18 healthy adult Beagle dogs aged(2.50±0.56)years were randomly divided into 3 groups with 6 replicates per group and 1 dog per replicate.The three groups were control group(CK group)and two probiotic addition groups(LR group and HR group),in which the CK group was fed a basal diet,while the LR group and HR group were fed the basal diet supplemented with 1×10^(8)and 1×10^(10)CFU/mL canine-derived Lactobacillus reuteri LRA7,respectively,and each dog was given 2 mL oral administration per day.The pre-trial period lasted for 7 days and the experimental period lasted for 28 days.The results showed as follows:1)acetic and propionic acid contents in feces was significantly increased in the LR and HR groups compared with the CK group(P<0.05).2)There was no significant difference in α-diversity and β-diversity of intestinal flora between the groups(P>0.05).3)Analysis of the genus level species abundance diagram showed that,compared with the CK group,the relative abundance of the Lactobacillus in the probiotic-added group increased,but there was no significant difference(P>0.05).The results of the t-test and LEfSe differential analysis of the intestinal flora showed that the relative abundances of the Bacteroidota,Prevotella and Veillonellaceae in the LR group increased,while the relative abundance of the Clostridium decreased(P<0.05).4)Compared with the CK group,the differential metabolites of dogs in the LR group were mainly annotated to the amino acid metabolic pathway,and those of dogs in the HR group were mainly annotated to the lipid metabolic pathway.In summary,dietary supplementation with Lactobacillus reuteri LRA7 can increase feces acetic acid and propionic acid contents,improve intestinal flora composition,and significantly up-regulate amino acid metabolic pathway and lipid metabolic pathway,which improves the intestinal heal

关 键 词：犬 罗伊氏乳杆菌 短链脂肪酸 肠道菌群 代谢组 

分 类 号：S829.2[农业科学—畜牧学]