2型糖尿病对大鼠正畸牙移动与张力侧骨微结构参数的影响  

Effect of type 2 diabetes mellitus on orthodontic tooth movement and bone microstructure parameters on the tension side in rats

作　　者：鄢成波 罗秋池 樊佳兵 顾叶婷 邓倩 张军梅 Yan Chengbo;Luo Qiuchi;Fan Jiabing;Gu Yeting;Deng Qian;Zhang Junmei(School of Stomatology,Guizhou Medical University,Guiyang 550001,Guizhou Province,China;Department of Orthodontics 2,Affiliated Stomatological Hospital of Guizhou Medical University,Guiyang 550001,Guizhou Province,China)

机构地区：[1]贵州医科大学口腔医学院,贵州省贵阳市550001 [2]贵州医科大学附属口腔医院正畸二科,贵州省贵阳市550001

出　　处：《中国组织工程研究》2026年第4期824-831,共8页Chinese Journal of Tissue Engineering Research

基　　金：贵州省卫生健康委项目(gzwkj2023-61),项目负责人:张军梅。

摘　　要：背景:正畸牙移动的生物学基础是牙周组织的重塑,2型糖尿病可导致颌骨及牙槽骨代谢改变,故推测高糖环境下牙移动特性可能发生变化。目的:探究一个牙移动周期内2型糖尿病对大鼠正畸牙移动的影响。方法:选用72只雄性SD大鼠,随机挑选40只高脂饲料喂养4周后腹腔注射链脲佐菌素建立2型糖尿病模型,从建模成功大鼠中挑选32只随机分为糖尿病组(n=16)和糖尿病正畸组(n=16),其余32只大鼠随机分为对照组(n=16)和正畸组(n=16)。糖尿病正畸组、正畸组大鼠装配镍钛拉簧正畸加力装置,以50 g力近中移动单侧上颌第一磨牙,建立正畸牙移动模型。正畸后第3,7,14,21天麻醉后处死大鼠,利用Micro-CT检测大鼠第一磨牙近中移动距离及张力侧骨微结构参数变化。结果与结论:(1)正畸第3,7,14,21天,4组间牙移动距离比较差异有显著性意义(P<0.05);正畸第7,14,21天,4组间张力侧牙槽骨骨密度、骨体积分数、骨小梁分离度比较差异均有显著性意义(P<0.05);正畸第3,14天,4组间骨小梁厚度比较差异有显著性意义(P<0.05);其中,糖尿病正畸组正畸第21天的张力侧牙槽骨骨密度、骨体积分数、骨小梁厚度最小,牙移动距离、骨小梁分离度最大;(2)结果表明,2型糖尿病对大鼠正畸牙移动中张力侧骨微结构参数有不良影响,呈现骨质疏松状态。BACKGROUND:Bone remodeling is the biological basis of orthodontic tooth movement.Type 2 diabetes mellitus leads to metabolic changes in the jaw and alveolar bone,so it is hypothesized that tooth mobility characteristics may be altered in a high-sugar environment.OBJECTIVE:To explore the impact of type 2 diabetes mellitus on orthodontic tooth movement in rats within one tooth movement cycle.METHODS:Seventy-two Sprague-Dawley rats were selected.Forty rats were randomly chosen and fed with a high-fat diet to construct a type 2 diabetes mellitus model.Thirty-two rats that were successfully modeled were randomly divided into a type 2 diabetes mellitus group(n=16)and a diabetic orthodontic group(n=16).The remaining 32 rats were randomly divided into a control group(n=16)and an orthodontic group(n=16).The rats in the orthodontic group and the diabetic orthodontic group were equipped with nickel-titanium coil spring orthodontic force application devices to move the unilateral maxillary first molars mesially with a force of 50 g.The rats were anesthetized and sacrificed on the 3rd,7th,14th,and 21st days after orthodontic treatment,and Micro-CT was used to measure the mesial displacement of the first molars and detect the changes in the bone microstructure parameters on the tension side.RESULTS AND CONCLUSION:There were significant differences in the tooth movement distances among the four groups of rats on the 3rd,7th,14th,and 21st days of orthodontic treatment(P<0.05).There were significant differences in bone mineral density,bone volume fraction and trabecular bone separation on the tension side among the four groups on the 7th,14th,and 21st days of orthodontic treatment(P<0.05).There were differences in the trabecular thickness among the four groups on the 3rd and 14th days of orthodontic treatment(P<0.05).The diabetic orthodontic group had the smallest tension-side alveolar bone mineral density,bone volume fraction,and trabecular thickness,and the largest tooth movement distance and trabecular separation on the 21st d

关 键 词：正畸牙移动 2型糖尿病 SD大鼠 MICRO-CT 骨微结构 工程化组织构建 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R587.1