肿瘤坏死因子α、核因子κB及iba-1在主动脉夹层模型小鼠海马组织中的表达及意义  

Expression and significance of tumor necrosis factor alpha,nuclear factor kappaB and ionized calcium binding adaptor molecule-1 in the hippocampus of mice with aortic dissection

作　　者：马红 丁雪苓 王琪[3] 侣慧 阿斯亚·阿不得斯木 程心怡 马翔[3] Ma Hong;Ding Xueling;Wang Qi;Lyu Hui;Asya Albusm;Cheng Xinyi;Ma Xiang(Department of Pathology,School of Basic Medicine,Xinjiang Medical University,Urumqi 830017,Xinjiang Uygur Autonomous Region,China;Department of Forensic Medicine,School of Basic Medicine,Xinjiang Medical University,Urumqi 830017,Xinjiang Uygur Autonomous Region,China;Cardiac Center,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]新疆医科大学基础医学院病理学教研室,新疆维吾尔自治区乌鲁木齐市830017 [2]新疆医科大学基础医学院法医学教研室,新疆维吾尔自治区乌鲁木齐市830017 [3]新疆医科大学第一附属医院心脏中心,新疆维吾尔自治区乌鲁木齐市830011

出　　处：《中国组织工程研究》2026年第4期858-863,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(82360090),项目负责人:马翔。

摘　　要：背景:目前主动脉夹层对海马损伤的研究甚少,而联合检测血清离子钙接头蛋白分子1、肿瘤坏死因子α和核因子κB在主动脉夹层中的表达未见报道。目的:观察主动脉夹层小鼠模型海马组织形态学变化,探讨主动脉夹层小鼠海马肿瘤坏死因子α、核因子κB及离子钙接头蛋白分子1的表达及意义。方法:选取16只3周龄健康雄性C57BL/6小鼠,随机分成对照组和主动脉夹层组,每组8只。主动脉夹层组小鼠在给予β-氨基丙腈饮水4周之后,埋入血管紧张素Ⅱ微渗透泵3 d,建立主动脉夹层小鼠模型;正常对照组小鼠保持正常的饮食和饮水。模型建立完成后,测量小鼠升主动脉最大直径,行主动脉组织苏木精-伊红染色、EVG染色评估成模率,采用酶联免疫吸附试验检测血清中炎性因子肿瘤坏死因子α、白细胞介素6的水平;解剖取海马进行苏木精-伊红染色观察海马区域病理变化;Western blot检测海马中肿瘤坏死因子α、核因子κB以及小胶质细胞标志物离子钙接头蛋白分子1的蛋白表达。结果与结论:(1)与对照组相比,主动脉夹层组小鼠升主动脉最大直径明显增粗;(2)苏木精-伊红染色显示,主动脉夹层组小鼠主动脉中层明显增厚,主动脉壁结构破坏、紊乱;CA1和CA3区神经元排列疏松,体积缩小,核固缩深染;(3)主动脉夹层组血清中炎性因子肿瘤坏死因子α、白细胞介素6水平较对照组升高(P<0.01);(4)主动脉夹层组海马肿瘤坏死因子α、核因子κB、磷酸化核因子κB、离子钙接头蛋白分子1蛋白表达较对照组增多(P<0.05);(5)结果表明,主动脉夹层模型小鼠海马神经元损伤,可能与小胶质细胞活化和肿瘤坏死因子α、核因子κB蛋白表达升高有关。BACKGROUND:Hippocampal injury caused by aortic coarctation has been poorly studied,and combined detection of tumor necrosis factorα,nuclear factorκB and ionized calcium binding adaptor molecule-1 expression in aortic dissection has not been reported.OBJECTIVE:To observe histomorphologic changes in the hippocampus of a mouse model of aortic dissection and investigate the expression and significance of tumor necrosis factor alpha,nuclear factor kappaB and ionized calcium binding adaptor molecule-1 in the hippocampus of aortic dissection mice.METHODS:Sixteen healthy 3-week-old male C57BL/6 mice were randomly divided into two groups:control group and aortic dissection group,with eight mice in each group.In the aortic dissection group,mice were givenβ-aminopropionitrile monofumarate as drinking water for 4 weeks,and the angiotensin II microinfiltration pump was then implanted to establish an animal model of aortic dissection.Mice in the control group were given normal diet and water.After the model was established,the maximum diameter of the ascending aorta was measured,hematoxylin-eosin staining and EVG staining were performed to evaluate the model formation rate,and the levels of inflammatory factors tumor necrosis factorαand interleukin 6 in serum were detected by enzyme-linked immunosorbent assay.The hippocampus was dissected and stained with hematoxylin-eosin to observe the pathological changes of the hippocampus in brain sections.The protein expression of tumor necrosis factorα,nuclear factorκB and ionized calcium binding adaptor molecule-1 was detected by western blot analysis.RESULTS AND CONCLUSION:(1)Compared with the control group,the maximum diameter of the ascending aorta in the aortic dissection group was significantly enlarged.(2)Hematoxylin-eosin staining of the aorta showed obvious thickening of the middle aorta and destruction and disorder of the aortic wall structure in mice.Neurons in the CA1 and CA3 regions of mice were sparsely arranged,reduced in size,and showed pyknosis with deeply stained

关 键 词：主动脉夹层 海马 神经元 肿瘤坏死因子Α Iba-1 工程化神经组织 

分 类 号：R496[医药卫生—康复医学] R-332[医药卫生—临床医学] R543.1