心俞穴位埋线预处理改善急性心肌缺血模型大鼠的心功能  

Thread embedding pretreatment at Xinshu(BL 15)improves cardiac function of acute myocardial ischemia rats

作　　者：陈小青 卞路瑶 陆星宇 杨涛 李湘海 Chen Xiaoqing;Bian Luyao;Lu Xingyu;Yang Tao;Li Xiang Hai(Guizhou Medical University,Guiyang 550004,Guizhou Province,China;Longchang People’s Hospital,Neijiang 642150,Sichuan Province,China;Department of Traditional Chinese Medicine,Affiliated Hospital of Guizhou Medical University,Guiyang 550004,Guizhou Province,China)

机构地区：[1]贵州医科大学,贵州省贵阳市550004 [2]隆昌市人民医院,四川省内江市642150 [3]贵州医科大学附属医院中医科,贵州省贵阳市550004

出　　处：《中国组织工程研究》2026年第4期882-891,共10页Chinese Journal of Tissue Engineering Research

基　　金：贵州省卫生健康委科学技术基金项目(gzwkj2024-291),项目负责人:李湘海;贵州医科大学附属医院国家自然科学基金面上项目培育计划项目(gyfynsfc[2023]-56),项目负责人:李湘海;贵州医科大学教学改革研究重点项目(JG2021012),项目负责人:李湘海。

摘　　要：背景:针刺心俞穴能显著改善急性心肌缺血的心功能,保护心肌细胞,但心俞穴位埋线处理对急性心肌缺血心功能的效果及机制尚不明确。核因子κB激活常出现P65亚型的核内易位,核因子κB信号通路的活化标志是P65水平升高。目的:探究心俞穴位埋线预处理对急性心肌缺血大鼠心功能及白细胞介素10、肿瘤坏死因子α、P65基因和蛋白表达水平的影响。方法:采用随机数字表法将32只雄性SD大鼠分为空白组、模型组、心俞穴组、非经非穴组,每组8只,后3组构建急性心肌缺血大鼠模型。心俞穴组大鼠心俞穴位埋线14 d,随后背部皮下注射盐酸异丙肾上腺素构建急性心肌缺血大鼠模型;非经非穴组局部埋线14 d,余同上;模型组心俞仅标记,余同上;空白组心俞仅标记,随后背部皮下注射等量生理盐水。造模后24 h检测心电图、心脏超声,腹主动脉取血ELISA法检测血清肌酸激酶和肌酸激酶同工酶水平,随后麻醉处死大鼠取材。苏木精-伊红和TUNEL染色观察心肌组织病理学变化和心肌细胞凋亡情况;RT-qPCR和Western Blot法检测心肌组织肿瘤坏死因子α、白细胞介素10、P65 mRNA及蛋白表达。结果与结论:(1)心电图:与空白组比较,模型组、非经非穴组、心俞穴组心电图Ⅱ导联ST段显著抬高;(2)心脏超声:与模型组比较,心俞穴组左室收缩末期内径显著减小(P<0.05),左室射血分数、左室短轴短缩率显著升高(P<0.05);(3)血清肌酸激酶和肌酸激酶同工酶:与模型组比较,心俞穴组显著降低(P<0.05);(4)苏木精-伊红染色:与模型组比较,心俞穴组心肌纤维排列基本整齐,水肿较轻,有少许炎性细胞浸润;(5)TUNEL染色:与模型组比较,心俞穴组心肌细胞凋亡荧光强度显著降低,凋亡率显著降低(P<0.05);(6)RT-qPCR、WesternBlot:与模型组比较,心俞穴组心肌组织白细胞介素10表达水平显著增高(P<0.05)、肿瘤坏死因子α、P65表达水平显�BACKGROUND:Acupuncture at Xinshu(BL 15)can significantly improve cardiac function and protect myocardial cells in acute myocardial ischemia,but the effect and mechanism of thread embedding treatment at Xinshu(BL 15)on cardiac function in acute myocardial ischemia are yet unclear.Nuclear factorκB activation often appears as an intranuclear translocation of the P65 isoform,and activation of the nuclear factorκB signaling pathway is marked by elevated P65 levels.OBJECTIVE:To explore the effects of thread embedding pretreatment at Xinshu(BL 15)on cardiac function and the expression levels of interleukin-10,tumor necrosis factor-α,P65 genes and proteins in rats with acute myocardial ischemia.METHODS:Thirty-two male Sprague-Dawley rats were randomly divided into a blank group,a model group,a Xinshu(BL 15)acupoint group,and a non-meridian/non-acupoint group using a random number table method,with eight rats in each group.Rat models of acute myocardial ischemia were established in the latter three groups.The Xinshu(BL 15)acupoint group had thread embedding at Xinshu(BL 15)for 14 days,followed by subcutaneous injection of isoproterenol hydrochloride into the back to establish an acute myocardial ischemia rat model.The non-meridian/non-acupoint group had local thread embedding for 14 days,and the rest procedures were the same as above.In the model group,Xinshu(BL 15)was only marked,and the rest procedures were the same as above.In the blank group,Xinshu(BL 15)was only marked,and then an equal amount of physiological saline was injected subcutaneously into the back.After 24 hours of modeling,electrocardiogram and cardiac ultrasound were performed.Abdominal aorta blood was extracted for detection of serum creatine kinase and creatine kinase isoenzyme levels using enzyme-linked immunosorbent assay.Subsequently,the rats were euthanized and samples were collected.Hematoxylin-eosin and TUNEL staining were used to observe the pathological changes of myocardial tissue and the apoptosis of myocardial cells.Real-time fluorescence

关 键 词：心俞 穴位埋线 急性心肌缺血 核因子κB 异丙肾上腺素 心功能 P65 白细胞介素10 工程化组织构建 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R245