高原低氧环境下仿野生黄芪和平栽黄芪水提物的抗疲劳作用及机制  

Anti-fatigue effect and mechanism of action of aqueous extracts of imitation wild Astragalus and cultivated Astragalus under high-altitude low oxygen environments

作　　者：朱云峰 边祥雨 常燕云 姚站馨 李爱平[2] 高蔚娜 ZHU Yunfeng;BIAN Xiangyu;CHANG Yanyun;YAO Zhanxin;LI Aiping;GAO Weina(Military Academy of Medical Sciences,Tianjin 300050,China;Modern Research Center for Traditional Chinese Medicine,Shanxi University,Taiyuan 030006,China)

机构地区：[1]军事科学院军事医学研究院,天津300050 [2]山西大学中医药现代研究中心,山西太原030006

出　　处：《中国药理学与毒理学杂志》2025年第4期241-251,共11页Chinese Journal of Pharmacology and Toxicology

摘　　要：目的探究仿野生黄芪和平栽黄芪水提物在模拟高原低压低氧环境下的抗疲劳作用及其相关机制。方法(1)将56只雄性昆明小鼠随机分为低氧游泳对照(HSC)组、仿野生黄芪水提物(IWA)430、860和1720 mg·kg^(-1)组和平栽黄芪水提物(CA)463、925和1850 mg·kg^(-1)组,ig给药,每天1次,连续15 d。每3 d记录小鼠体重。给药15 d后,各组在低压氧舱(模拟4000 m海拔高度)内负重游泳,记录力竭游泳时间,筛选最佳给药剂量。(2)将50只雄性昆明小鼠随机分为常氧对照(NC)组、低氧对照(HC)组、HSC组、IWA860 mg·kg^(-1)和CA 925 mg·kg^(-1)组,ig给药,每天1次,连续15 d。每3 d记录小鼠体重。给药15 d后,HSC组、IWA 860组和CA 925 mg·kg^(-1)组分别在低压氧舱内进行90 min非负重游泳,进行肌力测定。HE染色观察肝组织和腓肠肌组织病理变化;采用试剂盒测定血清中尿素氮(BUN)、血糖(BG)和乳酸(LA)含量及肝和骨骼肌中谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、超氧化物歧化酶(SOD)、肝糖原(LG)和肌糖原(MG)含量以及骨骼肌组织中总抗氧化能力(T-AOC)和活性氧(ROS)含量;高效液相色谱法测定骨骼肌组织中牛磺酸和亚牛磺酸含量;ELISA法检测肝组织半胱氨酸亚磺酸脱羧酶(CSAD)含量;Western印迹法检测骨骼肌组织磷脂酰肌醇3激酶(PI3K)、蛋白激酶B(Akt)、核因子E2相关因子2(Nrf2)和血红素加氧酶1(HO-1)蛋白表达水平。结果(1)与HSC组相比,IWA 430 mg·kg^(-1)组、IWA 860 mg·kg^(-1)组、CA 925 mg·kg^(-1)组和CA 1850 mg·kg^(-1)组小鼠力竭游泳时间显著延长。(2)与HSC组相比,IWA860 mg·kg^(-1)组和CA 925 mg·kg^(-1)组小鼠肌力增加;肝和腓肠肌组织病理学损伤明显减轻;血清中LA和BUN含量下降,BG、HG和MG水平显著增加;肝组织中GSH、GSH-Px和SOD水平显著增加,MDA水平显著下降,CSAD表达显著增加;骨骼肌组织中GSH、T-AOC、牛磺酸及亚牛磺酸含量显著增加,ROS水平显著OBJECTIVE To study the anti-fatigue effects of differently-cultivated Astragalus extract in a hypoxic environment of the plateau and explore the related mechanisms.METHODS Fifty-six male KM mice were randomly divided into the hypoxic swimming control(HSC)group,imitation wild Astragalus extract(IWA)430,860 and 1720 mg·kg^(-1)groups,and cultivated Astragalus extract(CA)463,925 and 1850 mg·kg^(-1)groups.The drug was administered by gavage once daily for 15 days,while body mass was monitored every three days.After 15 days of gavage,the mice were subjected to load swimming(5%body weight)in a hypobaric chamber(simulating a 4000 m altitude),with exhaus⁃tive swimming time measured to identify the optimal dosage.Following randomization,fifty male KM mice were assigned to five groups:normoxic control(NC),hypoxic control(HC),HSC,IWA 860 and CA 925 mg·kg^(-1).All groups underwent daily gavage for 15 d before 90 min non-weight-bearing swimming was conducted in the HSC,IWA 860 and CA 925 mg·kg^(-1)groups within a hypobaric chamber,followed by immediate measurement of muscle strength.Hematoxylin-eosin(HE)staining was used to observe the histopathological changes in liver and gastrocnemius muscle tissues.Blood urea nitrogen(BUN),blood glucose(BG)and serum lactic acid(LA),glutathione(GSH),glutathione peroxidase(GSH-Px),malondialdehyde(MDA),superoxide dismutase(SOD),liver glycogen(LG)and muscle glycogen(MG)in livers and muscles,and total antioxidant capacity(T-AOC)and reactive oxygen species(ROS)in muscles were measured by commercial kits.Taurine and hypotaurine were measured by HPLC.Enzymelinked immunosorbent assay(ELISA)was used for cysteine sulfenic acid decarboxylase(CSAD)measure⁃ment.Western blotting was used to detect protein expressions of phosphatidylinositol 3-kinase(PI3K),protein kinase B(Akt),nuclear factor E2-related factor 2(Nrf2),and heme oxygenase-1(HO-1)in skeletal muscles.RESULTS Compared with the HSC group,the swimming time was prolonged in IWA 463,IWA 860,CA 925 and CA 1850 mg·kg^(-1)groups.Compared wit

关 键 词：黄芪水提物 低压低氧 氧化应激 抗疲劳 

分 类 号：R964[医药卫生—药理学] R285[医药卫生—药学]