抗虫转基因玉米对草地贪夜蛾的室内抗性水平鉴定  

Evaluation of Resistance of Insect-Resistant Transgenic Maize to Spodoptera frugiperda in Laboratory

作　　者：熊梅 宋君[1] 魏超[1] 李辉 杨强 代晓航[1] 刘文娟[1] XIONG Mei;SONG Jun;WEI Chao;LI Hui;YANG Qiang;DAI Xiao-hang;LIU Wen-juan(Institute of Quality Standard and Testing Technology Research,Sichuan Academy of Agricultural Sciences,Chengdu 610066,PRC)

机构地区：[1]四川省农业科学院农业质量标准与检测技术研究所,四川成都610066

出　　处：《湖南农业科学》2025年第3期61-66,共6页Hunan Agricultural Sciences

基　　金：农业生物育种重大专项(2023ZD04062);四川省农业科学院“1+9”揭榜挂帅科技攻关项目(1+9 KJGG 006)。

摘　　要：分别以转Cry1Ab基因和转Vip3Aa20基因的转基因玉米为供体,通过传统杂交育种的方式,将Cry1Ab基因和Vip3Aa20基因转入先达901玉米中,采用室内离体组织生物测定的方法来评价抗虫转基因玉米对草地贪夜蛾的抗性水平,采用酶联免疫方法检测外源蛋白含量,生测结果与蛋白检测结果相互印证。结果表明:转基因玉米先达901在各时期各组织中均能稳定高剂量表达外源蛋白Cry1Ab和Vip3Aa20,且对草地贪夜蛾的致死作用强,具有较好的商业化应用前景。With the transgenic maize carrying Cry1Ab and that carrying Vip3Aa20 as donors,Cry1Ab and Vip3Aa20 were introduced into the maize cultivar'Xianda 901'by cross breeding.The resistance of insect-resistant transgenic maize to Spodoptera frugiperda was evaluated by in vitro tissue bioassay,and the content of exogenous proteins was determined by enzyme-linked immunosorbent assay.The results of bioassay and protein detection confirmed each other.The results showed that transgenic'Xianda 901'stably expressed exogenous proteins Cry1Ab and Vip3Aa20 at high levels in all tissues at all stages and had strong lethal effect on S.frugiperda,demonstrating a promising commercial application prospect.

关 键 词：抗虫转基因玉米 草地贪夜蛾 室内抗性鉴定 Cry1Ab蛋白 Vip3Aa20蛋白 

分 类 号：S435.132[农业科学—农业昆虫与害虫防治]