补肾壮骨方对骨关节炎大鼠软骨细胞外基质稳态的影响  

The effect of Bushen Zhuanggu Decoction on the extracellular matrixhomeostasis of chondrocytes in osteoarthritis rats

作　　者：张辽 傅科上 邓颖萍 金甬 吴权 史燕红 ZHANG Liao;FU Keshang;DENG Yingping;JIN Yong;WU Quan;SHI Yanhong(Department of Orthopaedics,Ningbo Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical University,Ningbo,Zhejiang 315000,China;不详)

机构地区：[1]浙江中医药大学附属宁波市中医院中医骨伤科,浙江宁波315000 [2]浙江中医药大学附属宁波市中医院肾病科

出　　处：《中华全科医学》2025年第5期760-764,共5页Chinese Journal of General Practice

基　　金：国家中医药管理局科技司-浙江省中医药管理局共建科技计划项目重点项目(GZY-ZJ-KJ-23037);浙江省中医药科技计划项目(2024ZL144);宁波市自然科学基金项目(2021J300)。

摘　　要：目的 探讨补肾壮骨方对大鼠软骨细胞外基质稳态失衡的影响及作用机制。方法 将36只大鼠采用随机数字表法分为6组,即假手术组、模型组、补肾壮骨方低浓度组、中浓度组、高浓度组和阳性对照组,每组6只。采用HE染色和番红O染色检测软骨组织病理学变化;检测Wnt/β-catenin信号通路相关蛋白及软骨退化相关蛋白(蛋白质印迹法)和mRNA(RT-PCR法)表达。结果 模型组软骨表层明显纤维化,软骨细胞减少,低、中、高浓度组和阳性对照组软骨细胞排列整齐,且高剂量组软骨病理学变化最显著。与模型组[(12.49±1.34)分]相比,低、中、高浓度组和阳性对照组大鼠改良Mankin's评分[分别为(10.17±1.19)分、(7.50±0.89)分、(3.99±0.45)分、(6.51±0.73)分]下降,软骨组织中IL-1β、Wnt5α、β-catenin、GSK-3β、MMP-13、ADAMTS5蛋白及mRNA表达明显降低,Axin、CollagenⅡ、Aggrecan蛋白和mRNA表达均明显升高(P<0.05)。结论 补肾壮骨方能抑制关节炎大鼠软骨细胞外基质降解,发挥治疗作用,其作用机制可能和抑制Wnt/β-catenin信号通路相关。Objective To investigate the effect and mechanism of Bushen Zhuanggu Decoction on the imbalance of extracellular matrix homeostasis in chondrocytes of osteoarthritis(OA)rats.Methods Thirty-six rats were randomly divided into six groups using a random number table:sham group,model group,Bushen Zhuanggu Decoction low-dose,medium-dose,high-dose group,and positive control group,with six in each group.Histological changes in cartilage tissue were evaluated using HE and Safranin O staining.The expression of proteins and mRNA related to the Wnt/β-catenin signaling pathway and cartilage degradation was assessed using Western blotting and RT-PCR,respectively.Results The model group exhibited significant cartilage surface fibrosis and reduced chondrocyte numbers.In contrast,the low-,medium-,and high-dose Bushen Zhuanggu Decoction groups,as well as the positive control group,showed an orderly arrangement of chondrocytes,with the most pronounced pathological improvement observed in the high-dose group.Compared to the model group(12.49±1.34),the Mankin's scores significantly decreased in the low-dose(10.17±1.19),medium-dose(7.50±0.89),high-dose(3.99±0.45),and positive control groups(6.51±0.73).The expression levels of IL-1β,Wnt5α,β-catenin,GSK-3β,MMP-13,and ADAMTS5 proteins and mRNA in cartilage tissue were significantly reduced,while the expression of Axin,CollagenⅡ,and Aggrecan proteins and mRNA were significantly increased(P<0.05).Conclusion Bushen Zhuanggu Decoction inhibits the degradation of the extracellular matrix in chondrocytes of OA rats and exerts therapeutic effects.Its mechanism may be related to the inhibition of the Wnt/β-catenin signaling pathway.

关 键 词：骨关节炎 补肾壮骨方 WNT/Β-CATENIN信号通路 软骨细胞外基质 

分 类 号：R684.3[医药卫生—骨科学] R-332[医药卫生—外科学]