非经典泛素化修饰的机制及其生物学功能  

The mechanism and biological function of non-canonical ubiquitination

作　　者：陈文静 戴京 闫明 叶茂[1,2] Wenjing Chen;Jing Dai;Ming Yan;Mao Ye(School of Biology,Hunan University,Changsha 410082,China;State Key Laboratory of Chemo-Biosensing and Metrology,Changsha 410082,China)

机构地区：[1]湖南大学生物学院,长沙410082 [2]化学生物传感与计量学国家重点实验室,长沙410082

出　　处：《中国科学:化学》2025年第4期847-858,共12页SCIENTIA SINICA Chimica

基　　金：国家重点研发计划(编号:2021YFA0909400);国家自然科学基金(编号:92253201,32350026,22334005)资助项目。

摘　　要：泛素化修饰是一种依赖于能量消耗来对底物进行特异性修饰的翻译后修饰方式,因其能够修饰众多的底物蛋白而在细胞的各项生命活动中发挥重要作用.随着对经典泛素化研究的深入,非经典泛素化修饰逐渐被发现,并引起广泛关注.非经典泛素化是一种新型的泛素修饰方式,其在修饰过程中底物氨基酸残基的种类、泛素与底物连接的连接方式、底物类型、酶促反应过程等方面均不同于经典泛素化修饰.非经典泛素化修饰可参与调节宿主-病原体互作、自噬、内质网相关降解、神经元活动等众多生命活动.本综述总结了非经典泛素化研究的最新进展,探讨了其在细胞中的作用机制和生物学功能,为进一步理解泛素化修饰的复杂性及其在生命过程中的重要性提供了新的视角.Ubiquitination is an energy-dependent post-translational modification that specifically modifies substrates.Due to its ability to modify numerous substrate proteins,it plays a crucial role in various cellular activities.With the deepening of research on canonical ubiquitination,non-canonical ubiquitination modifications have gradually been discovered and have attracted widespread attention.Non-canonical ubiquitination is a novel form of ubiquitin modification that differs from canonical ubiquitination in several aspects,including the types of amino acid residues of the substrate involved in the modification process,the linkage manner between ubiquitin and substrates,the types of substrates,and the enzymatic reaction processes.Non-canonical ubiquitination modifications can participate in regulating a multitude of biological activities such as host-pathogen interactions,autophagy,endoplasmic reticulumassociated degradation,and neuronal functions.This review summarizes the latest advancements in non-canonical ubiquitination research,discusses its mechanisms of action and biological functions within cells,and provides new perspectives for further understanding the complexity of ubiquitination modifications and their significance in life processes.

关 键 词：泛素化 非经典 翻译后修饰 机制 功能 

分 类 号：Q51[生物学—生物化学]