化学反应驱动的修饰核酸检测技术  

Chemical reaction-driven detection technologies for modified nucleic acids

作　　者：方振天 王雅芬 周翔[1] Zhentian Fang;Yafen Wang;Xiang Zhou(College of Chemistry and Molecular Sciences,Wuhan University,Wuhan 430072,China;School of Public Health,Wuhan University,Wuhan 430071,China)

机构地区：[1]武汉大学化学与分子科学学院,武汉430072 [2]武汉大学公共卫生学院,武汉430071

出　　处：《中国科学:化学》2025年第4期949-961,共13页SCIENTIA SINICA Chimica

基　　金：国家重点基础研究发展规划(编号:2023YFC3402200);国家自然科学基金(编号:92153303,22377096,22407105)。

摘　　要：修饰核酸在多种生命过程中发挥关键作用,如调节基因表达、调控RNA稳定性和影响蛋白质翻译等.深入理解修饰核酸的调控机制,有助于提升人们对基因相关疾病的认知,并促进新型诊疗策略的开发.为了探究这些修饰核酸的功能,研究者开发了各类结合高通量测序的检测技术.基于化学工具对修饰核酸进行定位和定性分析具有显著的优势,本文总结了近年来开发的化学反应驱动的修饰核酸检测技术,展示了化学工具在生物领域中的重要作用.Modified nucleic acids play a crucial role in various biological processes,such as regulating gene expression,controlling RNA stability,and influencing protein translation.A deeper understanding of the regulatory mechanisms of modified nucleic acids can enhance our knowledge of gene-related diseases and facilitate the development of novel diagnostic and therapeutic strategies.To explore the functions of these modified nucleic acids,researchers have developed various detection technologies integrated with high-throughput sequencing.The use of chemical tools for the localization and qualitative analysis of modified nucleic acids offers significant advantages.This review summarizes the chemical reaction-driven detection technologies for modified nucleic acids developed in recent years,highlighting the important role of chemical tools in the biological field.

关 键 词：核酸修饰 修饰检测 化学反应驱动 高通量测序 化学生物学 

分 类 号：Q503[生物学—生物化学]