蛋白质甲基化分析新技术研究进展  

Research progress in technologies for protein methylation analysis

作　　者：王科云[1,2] 李海涛 叶明亮[1,2] Keyun Wang;Haitao Li;Mingliang Ye(CAS Key Laboratory of Separation Science for Analytical Chemistry,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian 116023,China;University of Chinese Academy of Sciences,Beijing 100049,China;School of Basic Medical Sciences,Tsinghua University,Beijing 100084,China)

机构地区：[1]中国科学院大连化学物理研究所,中国科学院分离分析化学重点实验室,大连116023 [2]中国科学院大学,北京100049 [3]清华大学基础医学院,北京100084

出　　处：《中国科学:化学》2025年第4期962-976,共15页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(编号:92153302);国家重点研发计划(编号:2021YFA1302601)资助项目。

摘　　要：蛋白质甲基化修饰在细胞生理过程中发挥着重要的调控作用.为实现蛋白质甲基化的深入研究,发展高效的分析技术十分关键.随着蛋白组学技术的不断完善发展,蛋白质甲基化的高通量研究已取得了巨大的突破.蛋白质甲基化分析技术的研究大致包括:甲基化修饰位点的鉴定分析、甲基转移酶的催化机制研究和蛋白质甲基化相关的相互作用分析.近年来,在这些研究领域,相关的分析技术都取得了显著的进展,本评述从上述方面展开,介绍了蛋白质甲基化研究的最新进展情况.Protein methylation plays an extremely important role in regulating physiological processes.Development of efficient analytical techniques is crucial for the in-depth analysis of protein methylation.With the continuous improvement and development of proteomics technology,there have been significant breakthroughs in the highthroughput analysis of protein methylation.The study of protein methylation includes the identification of methylation sites,analysis of the catalytic mechanism of methyltransferases,and research on the regulatory functions of protein methylation.In recent years,significant progress has been made in the analytical techniques in these research fields.This review will describe these recent advancements in protein methylation research from the above aspects.

关 键 词：蛋白组学 蛋白质甲基化 翻译后修饰 甲基转移酶 

分 类 号：Q51[生物学—生物化学]